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Effect of cigarette smoking on paraoxonase 1 activity according to PON1 L55M and PON1 Q192R gene polymorphisms
- Source :
- Environmental Health and Preventive Medicine. 17:316-321
- Publication Year :
- 2011
- Publisher :
- Springer Science and Business Media LLC, 2011.
-
Abstract
- This study aims to investigate the effect of cigarette smoking on paraoxonase 1 (PON1) activity according to PON1 L55M and PON1 Q192R gene polymorphisms. Our sample included 300 voluntary subjects: 138 nonsmokers and 162 current smokers aged 38.47 ± 21.91 and 35.55 ± 16.03 years, respectively. PON1 activity was determined by kinetic methods. L55M and Q192R gene polymorphisms of PON1 were determined by multiplex polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP). We found in smokers a significant decrease of PON1 activity before and after adjustment. We noted a significant association between smoking status and lower PON1 activity [odds ratio (OR) = 3.03, confidence interval 95% = 1.5–5.9, p = 0.001]. In smokers, there was significant association between PON1 activity and PON1 L55M polymorphisms (p = 0.01). Also, the 55MM genotype presented the lowest paraoxonase activity, while the 55LL genotype showed the highest one. After adjustment for confounding variables, smokers with PON1 L55M polymorphism had the highest risk for lower PON1 activity; however, PON1 Q192R genotype might protect smokers from decrease in PON1 activity. We found significant interaction between the effect of cigarette smoking and both PON1 L55M and PON1 Q192R polymorphisms on lower PON1 activity. Cigarette smoking was significantly associated with decrease in PON1 activity. Moreover, PON1 L55M polymorphism predisposes smokers to decreased PON1 activity in contrast to PON1 Q192R genotype.
- Subjects :
- Adult
Male
Risk
medicine.medical_specialty
Tunisia
Biology
Young Adult
Polymorphism (computer science)
Internal medicine
Genotype
medicine
Humans
Cotinine
Polymorphism, Genetic
Aryldialkylphosphatase
Smoking
Confounding
Public Health, Environmental and Occupational Health
Paraoxonase
Regular Article
General Medicine
Odds ratio
Middle Aged
PON1
Molecular biology
Confidence interval
Logistic Models
Endocrinology
Multivariate Analysis
biology.protein
Female
Restriction fragment length polymorphism
Multiplex Polymerase Chain Reaction
Biomarkers
Polymorphism, Restriction Fragment Length
Thiocyanates
Subjects
Details
- ISSN :
- 13474715 and 1342078X
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- Environmental Health and Preventive Medicine
- Accession number :
- edsair.doi.dedup.....ce755336f6526ee0c82819dec1183311