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P-gp expression levels in the erythrocytes of brown trout: a new tool for aquatic sentinel biomarker development
- Source :
- Biomarkers, Biomarkers, Taylor & Francis, 2017, 22 (6), pp.566-574. ⟨10.1080/1354750X.2017.1338314⟩, Biomarkers, 2017, 22 (6), pp.566-574. ⟨10.1080/1354750X.2017.1338314⟩
- Publication Year :
- 2017
- Publisher :
- Informa UK Limited, 2017.
-
Abstract
- P-glycoprotein (P-gp) is a ubiquitous membrane detoxification pump involved in cellular defence against xenobiotics. Blood is a hub for the trade and transport of physiological molecules and xenobiotics. Our recent studies have highlighted the expression of a 140-kDa P-gp in brown trout erythrocytes in primary cell culture and its dose-dependent response to Benzo[a]pyrene pollutant.The purpose of this study was focused on using P-gp expression in brown trout erythrocytes as a biomarker for detecting the degree of river pollution.abcb1 gene and P-gp expression level were analysed by reverse transcriptase-PCR and Western blot, in the erythrocytes of brown trouts. The latter were collected in upstream and downstream of four rivers in which 17 polycyclic aromatic hydrocarbons and 348 varieties of pesticides micro-residues were analysed by liquid chromatography and mass spectrometry.The abcb1 gene and the 140-kDa P-gp were not expressed in trout erythrocytes from uncontaminated river. In contrast, they are clearly expressed in contaminated rivers, in correlation with the river pollution degree and the nature of the pollutants.This biological tool may offer considerable advantages since it provides an effective response to the increasing need for an early biomarker.
- Subjects :
- 0301 basic medicine
Trout
Health, Toxicology and Mutagenesis
Clinical Biochemistry
Gene Expression
[SDV.CAN]Life Sciences [q-bio]/Cancer
010501 environmental sciences
Biology
01 natural sciences
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
Brown trout
brown trout
Rivers
Detoxification
Water Pollution, Chemical
Animals
ATP Binding Cassette Transporter, Subfamily B, Member 1
Polycyclic Aromatic Hydrocarbons
0105 earth and related environmental sciences
Pesticide Residues
river pollution
Biomarker
Blood
030104 developmental biology
chemistry
13. Climate action
Cell culture
Environmental chemistry
erythrocytes
P-gp
biomarker
Xenobiotic
River pollution
Biomarkers
Environmental Monitoring
Subjects
Details
- ISSN :
- 13665804 and 1354750X
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Biomarkers
- Accession number :
- edsair.doi.dedup.....cea35846a53a48c71e9a6b9ccc11983d