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Comparison of vesicular stomatitis virus pseudotyped with the S proteins from a porcine and a human coronavirus
- Source :
- Digital.CSIC. Repositorio Institucional del CSIC, instname
- Publication Year :
- 2009
- Publisher :
- Society for General Microbiology, 2009.
-
Abstract
- The surface proteins S of severe acute respiratory syndrome coronavirus (SARS-CoV) and transmissible gastroenteritis virus (TGEV) were compared for their ability to mediate infection of viral pseudotypes based on vesicular stomatitis virus (VSV). The cell tropism of the respective pseudotypes corresponded to the tropism of the viruses from which the S protein was derived. Higher infectivity values were obtained with the SARS-CoV S protein than with the TGEV S protein. Differences were observed with respect to the importance of the cytoplasmic tail and the membrane anchor of the S proteins. In the case of the SARS-CoV S protein, truncation of the cytoplasmic tail resulted in increased infectivity. For the TGEV S protein, the inactivation of an intracellular retention signal in the cytoplasmic tail was required. Exchange of the membrane anchor of the S proteins led to a low infection efficiency. Our results indicate that related glycoproteins may show substantial differences in their ability to mediate pseudotype infection. © 2009 SGM.<br />This work was supported by grants to G. H. from Deutsche Forschungsgemeinschaft (SFB621), the German Ministry of Education and Research (project code ‘O¨ kologie und Pathogenese von SARS’), and the European Union.
- Subjects :
- Swine
viruses
medicine.disease_cause
Virus
Microbiology
Cell Line
Viral Envelope Proteins
Virology
Cricetinae
medicine
Animals
Humans
Mononegavirales
Tropism
Coronavirus
Sequence Deletion
Infectivity
chemistry.chemical_classification
Membrane Glycoproteins
biology
Virulence
Transmissible gastroenteritis virus
Vesiculovirus
Rhabdoviridae
biology.organism_classification
chemistry
Severe acute respiratory syndrome-related coronavirus
Vesicular stomatitis virus
Spike Glycoprotein, Coronavirus
Glycoprotein
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Digital.CSIC. Repositorio Institucional del CSIC, instname
- Accession number :
- edsair.doi.dedup.....ceafb924b11380f4e8104e3785897d96