Protein kinase C is involved in cholecystokinin octapeptide-induced proliferative action in rat glioma C6 cells

Chotecystoknin octapeptide (CCK-8) has been shown to stimulate DNA synthesis in rat glioma C 6 cells by activation of CCKB type receptors. However, the signalling pathways contributing to this proliferative action in C 6 cells have not been investigated thus far. This study demonstrated that stimulation of rat glioma C 6 cells with CCK-8S resulted in activation of protein kinase C isozymes βI, βII, γ and ζ. The participation of protein kinase C in the CCK-8S-induced effect on C 6 cell growth was demonstrated by measurement of [ 3 H]thymidine incorporation and estimation of cell number. The data indicate that CCK-8S stimulates growth in rat glioma C 6 cells by a protein kinase C-dependent mechanism.