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Streptomyces lonarensis sp. nov., isolated from Lonar Lake, a meteorite salt water lake in India
- Source :
- Antonie van Leeuwenhoek. 109:225-235
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- A novel alkaliphilic actinomycete, strain NCL716(T), was isolated from a soil sample collected from the vicinity of Lonar Lake, an alkaline salt water meteorite lake in Buldhana district of Maharashtra State in India. The strain was characterised using a polyphasic taxonomic approach which confirmed that it belongs to the genus Streptomyces. Growth was observed over a pH range of 7-11 at 28 °C. The cell wall was found to contain LL-diaminopimelic acid and traces of meso-diaminopimelic acid. The major fatty acid components were identified as iso-C16:0 (46.8 %), C17:1 (12.4 %), anteiso-C15:0 (5.1 %) and anteiso-C17:1 (4.8 %). The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol. The major menaquinones were determined to be MK-9 (H6) (70.3 %), MK-9 (H4) (15.5 %) and MK-9 (H8) (7.2 %). The G+C content of the DNA of the type strain was determined to be 71.4 mol %. The 16S rRNA gene sequence has been deposited in GenBank with accession number FJ919811. Although the 16S rRNA gene sequence analysis revealed that strain NCL716(T) shares >99 % similarity with that of Streptomyces bohaiensis strain 11A07(T), DNA-DNA hybridization revealed only 33.2 ± 3.0 % relatedness between them. Moreover, these two strains can be readily distinguished by some distinct phenotypic characteristics. Hence, on the basis of phenotypic and genetic analyses, it is proposed that strain NCL716(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces lonarensis sp. nov., is proposed. The type strain is NCL 716(T) (=DSM 42084(T) = MTCC 11708(T) = KCTC 39684(T)).
- Subjects :
- DNA, Bacterial
0301 basic medicine
Molecular Sequence Data
India
Sodium Chloride
Streptomyces bohaiensis
DNA, Ribosomal
Microbiology
Streptomyces
03 medical and health sciences
RNA, Ribosomal, 16S
Botany
Alkaliphile
Molecular Biology
Phylogeny
Soil Microbiology
chemistry.chemical_classification
Base Composition
biology
Strain (chemistry)
Accession number (library science)
Fatty Acids
Fatty acid
General Medicine
16S ribosomal RNA
biology.organism_classification
Lakes
030104 developmental biology
chemistry
Soil microbiology
Subjects
Details
- ISSN :
- 15729699 and 00036072
- Volume :
- 109
- Database :
- OpenAIRE
- Journal :
- Antonie van Leeuwenhoek
- Accession number :
- edsair.doi.dedup.....cedebc88433b07237064611f09c538ca