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High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy
- Source :
- Nature Communications, Vol 9, Iss 1, Pp 1-13 (2018), Nature Communications
- Publication Year :
- 2018
- Publisher :
- Nature Portfolio, 2018.
-
Abstract
- The fabrication of functional tissues is essential for clinical applications such as disease treatment and drug discovery. Recent studies have revealed that the mechanical environments of tissues, determined by geometric cell patterns, material composition, or mechanical properties, play critical roles in ensuring proper tissue function. Here, we propose an acoustophoretic technique using surface acoustic waves to fabricate therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels. Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors. The therapeutic effects of the fabricated vessel constructs are demonstrated in experiments using an ischemia mouse model by exhibiting the remarkable recovery of damaged tissue. Our study can be referenced to fabricate various types of artificial tissues that mimic the original functions as well as structures.<br />Engineering 3D tissues faces the challenge of adequate vascularisation for nutrient delivery and gas exchange deep inside the construct. Here the authors use surface acoustic waves to create an aligned array of blood vessels in a hyaluronic acid hydrogel and use it to improve function in a mouse hindlimb ischemia model.
- Subjects :
- 0301 basic medicine
Science
Cell
Ischemia
General Physics and Astronomy
Adipose tissue
02 engineering and technology
General Biochemistry, Genetics and Molecular Biology
Umbilical vein
Article
Hydrogel, Polyethylene Glycol Dimethacrylate
03 medical and health sciences
chemistry.chemical_compound
Paracrine signalling
Mice
Hyaluronic acid
medicine
Adipocytes
Human Umbilical Vein Endothelial Cells
otorhinolaryngologic diseases
Animals
Humans
Hyaluronic Acid
lcsh:Science
Vascular tissue
Mice, Inbred BALB C
Multidisciplinary
Tissue Engineering
Tissue Scaffolds
Chemistry
Stem Cells
technology, industry, and agriculture
General Chemistry
021001 nanoscience & nanotechnology
medicine.disease
Coculture Techniques
Cell biology
030104 developmental biology
medicine.anatomical_structure
Sound
Blood Vessels
lcsh:Q
Female
Stem cell
0210 nano-technology
Subjects
Details
- Language :
- English
- ISSN :
- 20411723
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....cf2d21fe7f61c7573de8d8b12d08ef6c