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Evaluation of the Expression and Function of the TRE2-like and TRE2 Genes in Ecdysis of Harmonia axyridis
- Source :
- Frontiers in Physiology, Vol 10 (2019), Frontiers in Physiology
- Publication Year :
- 2019
- Publisher :
- Frontiers Media SA, 2019.
-
Abstract
- Harmonia axyridis is an important predatory insect and widely used in biological control of agricultural and forestry pests. Trehalose is directly involved in the energy storage of the H. axyridis and in the oxidative function of various physiological activities thereby providing an energy source for its growth and development. The aim of this study was to explore the potential function of membrane-bound-like trehalase (TRE2-like) and membrane-bound trehalase (TRE2) genes in H. axyridis by RNAi. In addition, the activity of soluble and membrane-bound trehalase and the expression of genes related to trehalose and glycogen metabolism were determined in the larvae injected with dsTRE2-like or dsTRE2. The results showed that wing abnormality and mortality appeared in adults, as well as the activity of soluble trehalase and glycogen contents increased when interfering with TRE2-like gene. However, the activity of membrane-bound trehalase, trehalose and glucose contents in the larvae decreased. The expression of glycogen synthase (GS) and glycogen phosphorylase (GP) genes were decreased after RNAi in the ecdysis stage. The expression of chitin synthase gene A (CHSA), chitin synthase gene B (CHSB), and trehalose-6-phosphate synthase genes (TPS) were decreased significantly after RNAi, especially in the ecdysis stage. These results indicated that RNA interference is capable of knocking down gene expression of TRE2-like and TRE2, thereby disrupting trehalose metabolism which affects the chitin synthesis pathway in turn and also leads to developmental defects, such as wing deformities. This study could provide some theoretical guidance for the function of TRE2 gene in other insects.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Harmonia axyridis
Physiology
Biology
01 natural sciences
lcsh:Physiology
physiological activities
03 medical and health sciences
chemistry.chemical_compound
Glycogen phosphorylase
RNA interference
Physiology (medical)
Gene expression
Trehalase
Glycogen synthase
Original Research
Glycogen
lcsh:QP1-981
TRE2-like
fungi
Chitin synthase
Trehalose
TRE2
Cell biology
010602 entomology
030104 developmental biology
chemistry
biology.protein
Energy source
Subjects
Details
- Language :
- English
- ISSN :
- 1664042X
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Frontiers in Physiology
- Accession number :
- edsair.doi.dedup.....cf4d75ed473baaf927f0a4c518f7319e
- Full Text :
- https://doi.org/10.3389/fphys.2019.01371