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Differential expression and localisation of TGF-β isoforms and receptors in the murine epididymis
- Source :
- Scientific Reports, Scientific Reports, Nature Publishing Group, 2020, 10 (1), ⟨10.1038/s41598-020-57839-5⟩, Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020), Scientific Reports, Nature Publishing Group, 2020, 10 (1), pp.995. ⟨10.1038/s41598-020-57839-5⟩, Scientific Reports, 2020, 10 (1), pp.995. ⟨10.1038/s41598-020-57839-5⟩, Scientific Reports, 2020, 10, ⟨10.1038/s41598-020-57839-5⟩
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Testes produce spermatozoa that transit through and are stored in the epididymis where they acquire their fertilising capacities. Spermatozoa appear in the genital tract at puberty, long after the immune system was trained to self-antigens. As a consequence, this organ has to set strategies to tolerate sperm antigens to avoid autoimmune responses that would specifically target and destroy them. A recent study pointed the Transforming Growth Factor-beta (TGF-β) signalling in the dendritic cells as a crucial mechanism for epididymal tolerance to spermatozoa. In the mouse, TGF-β exists under three isoforms, and three distinct receptors have been described. Using RT-qPCR, immunohistochemistry and ELISA techniques, we investigated the expression and spatial distribution of the epididymal TGF-β isoforms and of their receptors in young and adult mice. We showed that both ligands and receptors were produced by immune and non-immune cells in the epididymis, whatever the age mice have. These data bring new clues as to the mechanisms of peripheral tolerance to sperm cells in the murine epididymis and raise potential other implications of the cytokine isoforms.
- Subjects :
- Male
0301 basic medicine
Gene isoform
[SDV]Life Sciences [q-bio]
medicine.medical_treatment
lcsh:Medicine
Biology
[SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
Article
[SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction
Mice
03 medical and health sciences
0302 clinical medicine
Immune system
Antigen
Transforming Growth Factor beta
Testis
medicine
Animals
Protein Isoforms
lcsh:Science
Receptor
[SDV.BDD.GAM]Life Sciences [q-bio]/Development Biology/Gametogenesis
ComputingMilieux_MISCELLANEOUS
Epididymis
[SDV.BDD.GAM] Life Sciences [q-bio]/Development Biology/Gametogenesis
[SDV.BDLR.RS] Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction
030219 obstetrics & reproductive medicine
Multidisciplinary
lcsh:R
[SDV.BDD.EO] Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis
Peripheral tolerance
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
Spermatozoa
Cell biology
[SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis
030104 developmental biology
medicine.anatomical_structure
Cytokine
[SDV.IMM.IA]Life Sciences [q-bio]/Immunology/Adaptive immunology
Infertility
[SDV.IMM.IA] Life Sciences [q-bio]/Immunology/Adaptive immunology
lcsh:Q
Receptors, Transforming Growth Factor beta
Signal Transduction
Transforming growth factor
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....cf56e4de35694403cb7947e08cc80373