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Potential for false positive HIV test results with the serial rapid HIV testing algorithm
- Source :
- BMC Research Notes, Vol 5, Iss 1, p 154 (2012), Baveewo, Steven; Kamya, Moses R; Mayanja-Kizza, Harriet; Fatch, Robin; Bangsberg, David R; Coates, Thomas; et al.(2012). Potential for false positive HIV test results with the serial rapid HIV testing algorithm. BMC Research Notes, 5(1), 154. doi: http://dx.doi.org/10.1186/1756-0500-5-154. Retrieved from: http://www.escholarship.org/uc/item/9fq758b8, BMC Research Notes
- Publisher :
- Springer Nature
-
Abstract
- Background Rapid HIV tests provide same-day results and are widely used in HIV testing programs in areas with limited personnel and laboratory infrastructure. The Uganda Ministry of Health currently recommends the serial rapid testing algorithm with Determine, STAT-PAK, and Uni-Gold for diagnosis of HIV infection. Using this algorithm, individuals who test positive on Determine, negative to STAT-PAK and positive to Uni-Gold are reported as HIV positive. We conducted further testing on this subgroup of samples using qualitative DNA PCR to assess the potential for false positive tests in this situation. Results Of the 3388 individuals who were tested, 984 were HIV positive on two consecutive tests, and 29 were considered positive by a tiebreaker (positive on Determine, negative on STAT-PAK, and positive on Uni-Gold). However, when the 29 samples were further tested using qualitative DNA PCR, 14 (48.2%) were HIV negative. Conclusion Although this study was not primarily designed to assess the validity of rapid HIV tests and thus only a subset of the samples were retested, the findings show a potential for false positive HIV results in the subset of individuals who test positive when a tiebreaker test is used in serial testing. These findings highlight a need for confirmatory testing for this category of individuals.
- Subjects :
- Male
Human immunodeficiency virus (HIV)
lcsh:Medicine
HIV Infections
HIV Antibodies
medicine.disease_cause
Polymerase Chain Reaction
0302 clinical medicine
Medicine
Mass Screening
Uganda
030212 general & internal medicine
Young adult
False positive
lcsh:QH301-705.5
Medicine(all)
0303 health sciences
General Medicine
Qualitative PCR testing
3. Good health
Test (assessment)
Molecular Diagnostic Techniques
Christian ministry
Female
Algorithm
Algorithms
Research Article
Adult
False positive HIV test
Hiv testing
Sensitivity and Specificity
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Young Adult
mental disorders
Humans
False Positive Reactions
lcsh:Science (General)
Mass screening
HIV testing algorithm
030306 microbiology
business.industry
Biochemistry, Genetics and Molecular Biology(all)
Rapid diagnostic tests
lcsh:R
HIV
Reproducibility of Results
Positive HIV
lcsh:Biology (General)
DNA, Viral
Reagent Kits, Diagnostic
business
lcsh:Q1-390
Subjects
Details
- Language :
- English
- ISSN :
- 17560500
- Volume :
- 5
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Research Notes
- Accession number :
- edsair.doi.dedup.....cf57024ec0e6283d66f7379a49134470
- Full Text :
- https://doi.org/10.1186/1756-0500-5-154