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Regulation of apoptosis-related genes during interactions between oyster hemocytes and the alveolate parasite Perkinsus marinus
- Source :
- Fish & Shellfish Immunology. 83:180-189
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- The alveolate Perkinsus marinus is the most devastating parasite of the eastern oyster Crassostrea virginica. The parasite is readily phagocytosed by oyster hemocytes, but instead of intracellular killing and digestion, P. marinus can survive phagocytosis and divide in host cells. This intracellular parasitism is accompanied by a regulation of host cell apoptosis. This study was designed to gain a better understanding of the molecular mechanisms of apoptosis regulation in oyster hemocytes following exposure to P. marinus. Regulation of apoptosis-related genes in C. virginica, and apoptosis-regulatory genes in P. marinus, were investigated via qPCR to assess the possible pathways involved during these interactions. In vitro experiments were also carried out to evaluate the effect of chemical inhibitors of P. marinus antioxidant processes on hemocyte apoptosis. Results indicate the involvement of the mitochondrial pathway (Bcl-2, anamorsin) of apoptosis in C. virginica exposed to P. marinus. In parallel, the antioxidants peroxiredoxin and superoxide dismutase were regulated in P. marinus exposed to C. virginica hemocytes suggesting that apoptosis regulation in infected oysters may be mediated by anti-oxidative processes. Chemical inhibition of P. marinus superoxide dismutase resulted in a marked increase of reactive oxygen species production and apoptosis in infected hemocytes. The implication of oxygen-dependent apoptosis during P. marinus infection and disease development in C. virginica is discussed.
- Subjects :
- 0301 basic medicine
Oyster
Hemocytes
animal structures
Protozoan Proteins
Gene Expression
Apoptosis
Aquatic Science
Host-Parasite Interactions
Microbiology
Superoxide dismutase
03 medical and health sciences
0302 clinical medicine
Perkinsus marinus
biology.animal
Animals
Environmental Chemistry
Crassostrea
Perkinsus
chemistry.chemical_classification
Reactive oxygen species
biology
Caspase 3
Superoxide Dismutase
urogenital system
General Medicine
biology.organism_classification
030104 developmental biology
Proto-Oncogene Proteins c-bcl-2
chemistry
Alveolata
030220 oncology & carcinogenesis
biology.protein
sense organs
Reactive Oxygen Species
Eastern oyster
Peroxiredoxin
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- ISSN :
- 10504648
- Volume :
- 83
- Database :
- OpenAIRE
- Journal :
- Fish & Shellfish Immunology
- Accession number :
- edsair.doi.dedup.....cf68f4e7f2337882a33cd6cd5a958ab0