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Effect of membrane fusion protein AdeT1 on the antimicrobial resistance of Escherichia coli
- Source :
- Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020), Scientific Reports
- Publication Year :
- 2020
- Publisher :
- Nature Portfolio, 2020.
-
Abstract
- Acinetobacter baumannii is a prevalent pathogen that can rapidly acquire resistance to antibiotics. Indeed, multidrug-resistant A. baumannii is a major cause of hospital-acquired infections and has been recognised by the World Health Organization as one of the most threatening bacteria to our society. Resistance-nodulation-division (RND) type multidrug efflux pumps have been demonstrated to convey antibiotic resistance to a wide range of pathogens and are the primary resistance mechanism employed by A. baumannii. A component of an RND pump in A. baumannii, AdeT1, was previously demonstrated to enhance the antimicrobial resistance of Escherichia coli. Here, we report the results of experiments which demonstrate that wild-type AdeT1 does not confer antimicrobial resistance in E. coli, highlighting the importance of verifying protein production when determining minimum inhibitory concentrations (MICs) especially by broth dilution. Nevertheless, using an agar-based MIC assay, we found that propionylation of Lys280 on AdeT1 renders E. coli cells more resistant to erythromycin.
- Subjects :
- 0301 basic medicine
Acinetobacter baumannii
medicine.drug_class
Science
030106 microbiology
Antibiotics
Erythromycin
Microbial Sensitivity Tests
medicine.disease_cause
Antimicrobial resistance
Membrane Fusion
Article
Microbiology
03 medical and health sciences
Antibiotic resistance
Bacterial Proteins
Drug Resistance, Multiple, Bacterial
medicine
Escherichia coli
Multidisciplinary
biology
Membrane fusion protein
Lysine
Proteins
Membrane Proteins
biology.organism_classification
Recombinant Proteins
030104 developmental biology
Medicine
Efflux
Bacteria
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....cfbe503b4c3b4cb5e933bee48de44a94