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Downregulation of fascin in the first trimester placental villi is associated with early recurrent miscarriage
- Source :
- Experimental cell research. 403(1)
- Publication Year :
- 2021
-
Abstract
- Inadequate trophoblast proliferation, shallow invasion and exaggerated rate of trophoblast apoptosis are implicated in early recurrent miscarriage (ERM). However, the mechanistic bases of this association have not been fully established. We aimed at investigating the involvement of fascin, an actin-bundling protein, in trophoblast activities and ERM. We found that fascin was downregulated in the cytotrophoblasts (CTBs) and distal cytotrophoblasts (DCTs) of ERM placentae. Knockdown of fascin altered cellular and nucleolar morphology, and inhibited the proliferation but increased apoptosis of trophoblastic HTR8/SVneo cells. Furthermore, fascin knockdown decreased the expression of transcription factors such as Snail1/2, Twist and Zeb1/2, mesenchymal molecules such as Vimentin and N-cadherin, and the protein expression of phosphorylated extracellular signal-regulated kinase 1/2 (ERK1/2) and phosphorylates signal transducer and activator of transcript 3 (STAT3). Exposure of HTR-8/SVneo cells to hypoxia reoxygenation (H/R) decreased fascin expression to affect the cells' invasion. Our results indicate for the first time that the downregulation of fascin is involved in the pathogenesis of early recurrent miscarriage; and hence a potential therapeutic target against the disease.
- Subjects :
- 0301 basic medicine
Abortion, Habitual
Placenta
Down-Regulation
Vimentin
macromolecular substances
Pathogenesis
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
Cell Movement
Pregnancy
medicine
Humans
Phosphorylation
STAT3
reproductive and urinary physiology
Fascin
Cell Proliferation
Gene knockdown
biology
Microfilament Proteins
Trophoblast
Cell Biology
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
embryonic structures
biology.protein
Cancer research
Female
Cytotrophoblasts
Chorionic Villi
Carrier Proteins
Signal Transduction
Subjects
Details
- ISSN :
- 10902422
- Volume :
- 403
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Experimental cell research
- Accession number :
- edsair.doi.dedup.....cfd993c0c53352587b89903bfb040a95