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Co-delivery of Adenovirus and Carmustine by Anionic Liposomes with Synergistic Anti-tumor Effects

Authors :
Xun Sun
Yu Wan
Zhirong Zhong
Zhirong Zhang
Jianfeng Han
Sanjun Shi
Source :
Pharmaceutical Research. 29:145-157
Publication Year :
2011
Publisher :
Springer Science and Business Media LLC, 2011.

Abstract

To improve gene transducibility mediated by adenovirus (Ad) in cancer cells and further enhance anti-tumor effects by co-delivery.Calcium-induced phase change method was used to prepare the complex of anionic liposomes and adenovirus (AL/Ad5). Gene expression was qualitatively detected by X-gal staining and quantitatively detected by ELISA. Taking adenovirus-mediated stromal cell-derived factor-1α (Ad5-SDF1α) as therapeutic gene and carmustine (BCNU) as chemotherapeutic agent, a co-delivering system of AL/Ad5-SDF1α/BCNU was prepared and administered to tumor-bearing mice by intratumor injection.Enhanced LacZ gene transduction was obtained in B16 and Lewis lung carcinoma cells in vitro and in vivo. Complexes of AL/Ad5-SDF1α improved SDF1α gene expression and led to accumulation of dendritic cells among the murine B16 melanoma cells in vivo. This co-delivery system of AL/Ad5-SDF1α/BCNU could significantly suppress tumor growth and prolong survival of tumor-bearing mice.Through the co-delivering system, AL/Ad5-SDF1α could synergize with BCNU to improve the antitumor effect. It may be a promising strategy for solid tumor therapy.

Details

ISSN :
1573904X and 07248741
Volume :
29
Database :
OpenAIRE
Journal :
Pharmaceutical Research
Accession number :
edsair.doi.dedup.....cfee9eacc1680e4b7994b7f00867969b
Full Text :
https://doi.org/10.1007/s11095-011-0521-7