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Protein S is Protective in Acute Lung Injury by Inhibiting Cell Apoptosis
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, Vol 20, Iss 5, p 1082 (2019), Volume 20, Issue 5
- Publication Year :
- 2019
- Publisher :
- MDPI, 2019.
-
Abstract
- Acute lung injury is a fatal disease characterized by inflammatory cell infiltration, alveolar-capillary barrier disruption, protein-rich edema, and impairment of gas exchange. Protein S is a vitamin K-dependent glycoprotein that exerts anticoagulant, immunomodulatory, anti-inflammatory, anti-apoptotic, and neuroprotective effects. The aim of this study was to evaluate whether human protein S inhibits cell apoptosis in acute lung injury. Acute lung injury in human protein S transgenic and wild-type mice was induced by intratracheal instillation of lipopolysaccharide. The effect of human protein S on apoptosis of lung tissue cells was evaluated by Western blotting. Inflammatory cell infiltration, alveolar wall thickening, myeloperoxidase activity, and the expression of inflammatory cytokines were reduced in human protein S transgenic mice compared to the wild-type mice after lipopolysaccharide instillation. Apoptotic cells and caspase-3 activity were reduced while phosphorylation of extracellular signal-regulated kinase was enhanced in the lung tissue from human protein S transgenic mice compared to wild-type mice after lipopolysaccharide instillation. The results of this study suggest that human protein S is protective in lipopolysaccharide-induced acute lung injury by inhibiting apoptosis of lung cells.
- Subjects :
- 0301 basic medicine
Lipopolysaccharides
Male
Lipopolysaccharide
MAP Kinase Signaling System
Acute Lung Injury
Lung injury
Neuroprotection
Catalysis
Protein S
Article
Proinflammatory cytokine
lcsh:Chemistry
Inorganic Chemistry
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Erk1/2
Medicine
Animals
Humans
Physical and Theoretical Chemistry
lcsh:QH301-705.5
Molecular Biology
Spectroscopy
signal pathway
Lung
biology
business.industry
Kinase
Organic Chemistry
lipopolysaccharide
apoptosis
General Medicine
respiratory system
Computer Science Applications
Mice, Inbred C57BL
030104 developmental biology
medicine.anatomical_structure
lcsh:Biology (General)
lcsh:QD1-999
chemistry
Apoptosis
030220 oncology & carcinogenesis
biology.protein
Cancer research
business
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 20
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....d04c0d1815cd649f2d2a741c760c4525