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A septin from the filamentous fungus A. nidulans induces atypical pseudohyphae in the budding yeast S. cerevisiae
- Source :
- PLoS ONE, PLoS ONE, Vol 5, Iss 3, p e9858 (2010)
- Publication Year :
- 2009
-
Abstract
- Background Septins, novel cytoskeletal proteins, form rings at the bases of emerging round buds in yeasts and at the bases of emerging elongated hyphal initials in filamentous fungi. Methodology/Principal Findings When introduced into the yeast Saccharomyces cerevisiae, the septin AspC from the filamentous fungus Aspergillus nidulans induced highly elongated atypical pseudohyphae and spore-producing structures similar to those of hyphal fungi. AspC induced atypical pseudohyphae when S. cerevisiae pseudohyphal or haploid invasive genes were deleted, but not when the CDC10 septin gene was deleted. AspC also induced atypical pseudohyphae when S. cerevisiae genes encoding Cdc12-interacting proteins Bem4, Cla4, Gic1 and Gic2 were deleted, but not when BNI1, a Cdc12-interacting formin gene, was deleted. AspC localized to bud and pseudohypha necks, while its S. cerevisiae ortholog, Cdc12, localized only to bud necks. Conclusions/Significance Our results suggest that AspC competes with Cdc12 for incorporation into the yeast septin scaffold and once there alters cell shape by altering interactions with the formin Bni1. That introduction of the A. nidulans septin AspC into S. cerevisiae induces a shift from formation of buds to formation of atypical pseudohyphae suggests that septins play an important role in the morphological plasticity of fungi.
- Subjects :
- Heterozygote
Saccharomyces cerevisiae Proteins
Hypha
Saccharomyces cerevisiae
Green Fluorescent Proteins
Developmental Biology/Microbial Growth and Development
Hyphae
Cell Biology/Cell Growth and Division
lcsh:Medicine
macromolecular substances
Septin
Aspergillus nidulans
Microbiology
GTP Phosphohydrolases
03 medical and health sciences
Gene Expression Regulation, Fungal
Cell Biology/Cytoskeleton
lcsh:Science
Actin
Cytoskeleton
030304 developmental biology
0303 health sciences
Multidisciplinary
biology
030306 microbiology
Developmental Biology/Morphogenesis and Cell Biology
lcsh:R
fungi
Microfilament Proteins
Membrane Proteins
biology.organism_classification
Yeast
Actins
3. Good health
Cell biology
Phenotype
Membrane protein
Formins
Mutation
biology.protein
lcsh:Q
Cell Biology/Morphogenesis and Cell Biology
Gene Deletion
Septins
Plasmids
Research Article
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 5
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....d04f981fefce1a0f68bd8b1c8fa7265d