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A Proximity Labeling Strategy Provides Insights into the Composition and Dynamics of Lipid Droplet Proteomes
- Source :
- Dev Cell, Developmental cell, vol 44, iss 1, Developmental Cell
- Publication Year :
- 2017
-
Abstract
- Lipid droplet (LD) functions are regulated by a complement of integral and peripheral proteins that associate with the bounding LD phospholipid monolayer. Defining the composition of the LD proteome has remained a challenge due to the presence of contaminating proteins in LD-enriched buoyant fractions. To overcome this limitation, we developed a proximity labeling strategy that exploits LD-targeted APEX2 to biotinylate LD proteins in living cells. Application of this approach to two different cell types identified the vast majority of previously validated LD proteins, excluded common contaminating proteins, and revealed new LD proteins. Moreover, quantitative analysis of LD proteome dynamics uncovered a role forendoplasmic reticulum-associated degradation in controlling the composition of the LD proteome. These data provide an important resource for future LD studies and demonstrate the utility of proximity labeling to study the regulation of LD proteomes.
- Subjects :
- 0301 basic medicine
APEX2
Proteome
lipid droplet
Medical and Health Sciences
0302 clinical medicine
Lipid droplet
Receptors
biotinylation
APEX
biology
Autocrine Motility Factor
Membrane transport protein
Peripheral membrane protein
Endoplasmic Reticulum-Associated Degradation
Biological Sciences
endoplasmic reticulum
Biotinylation
proximity labeling
Biotechnology
endocrine system
Computational biology
Endoplasmic-reticulum-associated protein degradation
complex mixtures
General Biochemistry, Genetics and Molecular Biology
Article
03 medical and health sciences
ubiquitin
Humans
Molecular Biology
Staining and Labeling
Endoplasmic reticulum
technology, industry, and agriculture
Membrane Proteins
Membrane Transport Proteins
Cell Biology
Lipid Droplets
ERAD
eye diseases
Receptors, Autocrine Motility Factor
030104 developmental biology
proteasome
Proteasome
biology.protein
ATPases Associated with Diverse Cellular Activities
Generic health relevance
Carrier Proteins
030217 neurology & neurosurgery
Biomarkers
Developmental Biology
Subjects
Details
- ISSN :
- 18781551
- Volume :
- 44
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Developmental cell
- Accession number :
- edsair.doi.dedup.....d098b2a9c738cf25242fef1052c46bf4