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Evaluation of liquid from the Papanicolaou test and other liquid biopsies for the detection of endometrial and ovarian cancers
- Source :
- Wang, Y, Li, L, Douville, C, Cohen, J D, Yen, T T, Kinde, I, Sundfelt, K, Kjær, S K, Hruban, R H, Shih, I M, Wang, T L, Kurman, R J, Springer, S, Ptak, J, Popoli, M, Schaefer, J, Silliman, N, Dobbyn, L, Tanner, E J, Angarita, A, Lycke, M, Jochumsen, K, Afsari, B, Danilova, L, Levine, D A, Jardon, K, Zeng, X, Arseneau, J, Fu, L, Diaz, L A, Karchin, R, Tomasetti, C, Kinzler, K W, Vogelstein, B, Fader, A N, Gilbert, L & Papadopoulos, N 2018, ' Evaluation of liquid from the Papanicolaou test and other liquid biopsies for the detection of endometrial and ovarian cancers ', Science Translational Medicine, vol. 10, no. 433, 8793 . https://doi.org/10.1126/scitranslmed.aap8793, Wang, Y, Li, L, Douville, C, Cohen, J D, Yen, T T, Kinde, I, Sundfelt, K, Kjær, S K, Hruban, R H, Shih, I M, Wang, T L, Kurman, R J, Springer, S, Ptak, J, Popoli, M, Schaefer, J, Silliman, N, Dobbyn, L, Tanner, E J, Angarita, A, Lycke, M, Jochumsen, K, Afsari, B, Danilova, L, Levine, D A, Jardon, K, Zeng, X, Arseneau, J, Fu, L, Diaz, L A, Karchin, R, Tomasetti, C, Kinzler, K W, Vogelstein, B, Fader, A N, Gilbert, L & Papadopoulos, N 2018, ' Evaluation of liquid from the papanicolaou test and other liquid biopsies for the detection of endometrial and ovarian cancers ', Obstetrical and Gynecological Survey, vol. 73, no. 8, pp. 463-464 . https://doi.org/10.1097/01.ogx.0000542325.60681.a3
- Publication Year :
- 2018
-
Abstract
- We report the detection of endometrial and ovarian cancers based on genetic analyses of DNA recovered from the fluids obtained during a routine Papanicolaou (Pap) test. The new test, called PapSEEK, incorporates assays for mutations in 18 genes as well as an assay for aneuploidy. In Pap brush samples from 382 endometrial cancer patients, 81% [95% confidence interval (CI), 77 to 85%] were positive, including 78% of patients with early-stage disease. The sensitivity in 245 ovarian cancer patients was 33% (95% CI, 27 to 39%), including 34% of patients with early-stage disease. In contrast, only 1.4% of 714 women without cancer had positive Pap brush samples (specificity, ~99%). Next, we showed that intrauterine sampling with a Tao brush increased the detection of malignancy over endocervical sampling with a Pap brush: 93% of 123 (95% CI, 87 to 97%) patients with endometrial cancer and 45% of 51 (95% CI, 31 to 60%) patients with ovarian cancer were positive, whereas none of the samples from 125 women without cancer were positive (specificity, 100%). Finally, in 83 ovarian cancer patients in whom plasma was available, circulating tumor DNA was found in 43% of patients (95% CI, 33 to 55%). When plasma and Pap brush samples were both tested, the sensitivity for ovarian cancer increased to 63% (95% CI, 51 to 73%). These results demonstrate the potential of mutation-based diagnostics to detect gynecologic cancers at a stage when they are more likely to be curable.
- Subjects :
- 0301 basic medicine
Adult
medicine.medical_specialty
Adolescent
Aneuploidy
Papanicolaou stain
Malignancy
Gastroenterology
Article
03 medical and health sciences
Young Adult
0302 clinical medicine
Internal medicine
medicine
Humans
Stage (cooking)
Aged
Retrospective Studies
Ovarian Neoplasms
Vaginal Smears
030102 biochemistry & molecular biology
business.industry
Endometrial cancer
Liquid Biopsy
Obstetrics and Gynecology
Cancer
General Medicine
Papanicolaou Test
Middle Aged
medicine.disease
Endometrial Neoplasms
030104 developmental biology
030220 oncology & carcinogenesis
Female
Ovarian cancer
business
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Wang, Y, Li, L, Douville, C, Cohen, J D, Yen, T T, Kinde, I, Sundfelt, K, Kjær, S K, Hruban, R H, Shih, I M, Wang, T L, Kurman, R J, Springer, S, Ptak, J, Popoli, M, Schaefer, J, Silliman, N, Dobbyn, L, Tanner, E J, Angarita, A, Lycke, M, Jochumsen, K, Afsari, B, Danilova, L, Levine, D A, Jardon, K, Zeng, X, Arseneau, J, Fu, L, Diaz, L A, Karchin, R, Tomasetti, C, Kinzler, K W, Vogelstein, B, Fader, A N, Gilbert, L & Papadopoulos, N 2018, ' Evaluation of liquid from the Papanicolaou test and other liquid biopsies for the detection of endometrial and ovarian cancers ', Science Translational Medicine, vol. 10, no. 433, 8793 . https://doi.org/10.1126/scitranslmed.aap8793, Wang, Y, Li, L, Douville, C, Cohen, J D, Yen, T T, Kinde, I, Sundfelt, K, Kjær, S K, Hruban, R H, Shih, I M, Wang, T L, Kurman, R J, Springer, S, Ptak, J, Popoli, M, Schaefer, J, Silliman, N, Dobbyn, L, Tanner, E J, Angarita, A, Lycke, M, Jochumsen, K, Afsari, B, Danilova, L, Levine, D A, Jardon, K, Zeng, X, Arseneau, J, Fu, L, Diaz, L A, Karchin, R, Tomasetti, C, Kinzler, K W, Vogelstein, B, Fader, A N, Gilbert, L & Papadopoulos, N 2018, ' Evaluation of liquid from the papanicolaou test and other liquid biopsies for the detection of endometrial and ovarian cancers ', Obstetrical and Gynecological Survey, vol. 73, no. 8, pp. 463-464 . https://doi.org/10.1097/01.ogx.0000542325.60681.a3
- Accession number :
- edsair.doi.dedup.....d0dbc98b9316053a09f1adaf11a746b1