Back to Search
Start Over
Quantification of microcystin-producing and non-microcystin producing Microcystis populations during the 2009 and 2010 blooms in Lake Taihu using quantitative real-time PCR
- Source :
- Journal of Environmental Sciences. 24:284-290
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- Lake Taihu, a large, shallow hypertrophic freshwater lake in eastern China, has experienced lake-wide toxic cyanobacterial blooms annually during summer season in the past decades. Spatial changes in the abundance of hepatotoxin microcystin-producing and nonmicrocystin producing Microcystis populations were investigated in the lake in August of 2009 and 2010. To monitor the densities of the total Microcystis population and the potential microcystin-producing subpopulation, we used a quantitative real-time PCR assay targeting the phycocyanin intergenic spacer (PC-IGS) and the microcystin synthetase gene (mcyD), respectively. On the basis of quantification by real-time PCR analysis, the abundance of potential toxic Microcystis genotypes and the ratio of the mcyD subpopulation to the total Microcystis varied significantly, from 4.08 x 10(4) to 5.22 x 10(7) copies/mL, from 5.7% to 65.8%, respectively. Correlation analysis showed a strong positive relationship between chlorophyll-a, toxic Microcystis and total Microcystis; the abundance of toxic Microcystis correlated positively with total phosphorus and ortho-phosphate concentrations, but negatively with TN:TP ratio and nitrate concentrations. Meanwhile the proportion of potential toxic genotypes within Microcystis population showed positive correlation with total phosphorus and ortho-phosphate concentrations. Our data suggest that increased phosphorus loading may be a significant factor promoting the occurrence of toxic Microcystis bloom in Lake Taihu.
- Subjects :
- China
Veterinary medicine
Microcystis
Environmental Engineering
Genotype
Microcystins
Harmful Algal Bloom
Population
chemistry.chemical_element
Microcystin
Polymerase Chain Reaction
Algal bloom
Microbiology
chemistry.chemical_compound
Nitrate
Phycocyanin
Environmental Chemistry
education
General Environmental Science
chemistry.chemical_classification
education.field_of_study
biology
Phosphorus
Hepatotoxin
General Medicine
biology.organism_classification
Lakes
chemistry
Subjects
Details
- ISSN :
- 10010742
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Journal of Environmental Sciences
- Accession number :
- edsair.doi.dedup.....d0ed359b85b21994fd72346a0fa23061