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A Parvovirus B19 synthetic genome: sequence features and functional competence
- Publication Year :
- 2017
-
Abstract
- Central to genetic studies for Parvovirus B19 (B19V) is the availability of genomic clones that may possess functional competence and ability to generate infectious virus. In our study, we established a new model genetic system for Parvovirus B19. A synthetic approach was followed, by design of a reference genome sequence, by generation of a corresponding artificial construct and its molecular cloning in a complete and functional form, and by setup of an efficient strategy to generate infectious virus, via transfection in UT7/EpoS1 cells and amplification in erythroid progenitor cells. The synthetic genome was able to generate virus with biological properties paralleling those of native virus, its infectious activity being dependent on the preservation of self-complementarity and sequence heterogeneity within the terminal regions. A virus of defined genome sequence, obtained from controlled cell culture conditions, can constitute a reference tool for investigation of the structural and functional characteristics of the virus.
- Subjects :
- 0301 basic medicine
Infectious clone
viruses
Genome, Viral
Consensus sequence
Genomic clone
Parvovirus B19
Synthetic genome
Molecular cloning
Virus Replication
Genome
Virus
NO
Parvoviridae Infections
03 medical and health sciences
Virology
Genes, Synthetic
Parvovirus B19, Human
Humans
Sequence (medicine)
Whole genome sequencing
Genetics
biology
Parvovirus
Transfection
biology.organism_classification
030104 developmental biology
RNA, Viral
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....d0f6dd1a9d370fc7aa9f93a6d863acb1