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Statistical Validation of Two Sample Comparison Methods for Oligonucleotide Microarray in Rat Ischemia Model
- Source :
- Neurochemical Research. 31:735-740
- Publication Year :
- 2006
- Publisher :
- Springer Science and Business Media LLC, 2006.
-
Abstract
- In gene expression analyses using a high-density oligonucleotide array in a rat ischemia model, two comparison methods, "pair-wise comparison" and "sample average comparison", were evaluated based on statistical methods. The reliability of the elements screened with a 1.2 to 10-fold threshold was also evaluated. In pair-wise comparisons, most of the elements were significantly independent of the threshold value, with the percentage of significant elements remaining above 95%, when screened at 2.5-fold or higher threshold value. Pair-wise comparison structurally provided strict screening, which resulted in genes that were not selected despite significant alterations in expression. Screening by "sample average comparison" resulted in elements with low probability of significance, which suggested the necessity for increasing the reliability by additional statistical analyses after screening. When genes with altered expression were screened using an oligonucleotide array, marked differences in the numbers and reliability were proved to exist among elements screened by each sample comparison method.
- Subjects :
- Male
Microarray
Statistical validation
Ischemia
General Medicine
Computational biology
Biology
medicine.disease
Biochemistry
Molecular biology
Brain Ischemia
Rats
Disease Models, Animal
Cellular and Molecular Neuroscience
Oligonucleotide Microarray
Method comparison
medicine
Animals
Pairwise comparison
Two sample
Rats, Wistar
Reliability (statistics)
Oligonucleotide Array Sequence Analysis
Subjects
Details
- ISSN :
- 15736903 and 03643190
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Neurochemical Research
- Accession number :
- edsair.doi.dedup.....d103865f36abc4253979cfe7ec18e42b
- Full Text :
- https://doi.org/10.1007/s11064-006-9074-2