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The influence of ecto-nucleotidases on Leishmania amazonensis infection and immune response in C57B/6 mice
- Source :
- Repositório Institucional da UFOP, Universidade Federal de Ouro Preto (UFOP), instacron:UFOP
- Publication Year :
- 2009
-
Abstract
- Previous results from our laboratory and from the literature have implicated the expression of ecto-nucleotidases in the establishment of Leishmania infection. In the present study we evaluated the correlation between ecto-nucleotidasic activity and the infectivity of L. amazonensis promastigotes that were kept in culture for short or extended numbers of passages, a condition that is known to decrease parasite infectivity. We also analyzed the immune response associated with the infection by these parasites. As expected, we found that long-term cultured parasites induce the development of smaller lesions than the short-term cultured counterparts. Interestingly, long-term cultured parasites presented reduced ecto-nucleotidasic activity. In addition, cells recovered from animals infected with long-term cultured parasites produced higher amounts of IFN-gamma and have smaller parasite load, after 8weeks of infection. Furthermore, after 1week of infection, there is increased expression of the chemokine CCL2 mRNA in animals infected with short-term cultured parasites. Finally, infection of peritoneal macrophages by these parasites also shows marked differences. Thus, while short-term cultured parasites are able to infect a greater proportion of macrophages, cells infected by long-term cultured parasites express higher amounts of CXCL10 mRNA, which may activate these cells to kill the parasites. We suggest that the enzymes involved in metabolism of extracellular nucleotides may have an important role in infection by L. amazonensis, by acting directly in its adhesion to target cells and by modulating host cell chemokine production.
- Subjects :
- Chemokine
Adenosine
Macrophage
Virulence Factors
Veterinary (miscellaneous)
Protozoan Proteins
Biology
Parasite load
Microbiology
Interferon-gamma
Mice
Immune system
Interferon
Nucleotidases
medicine
CXCL10
Animals
Serial Passage
Leishmaniasis
Chemokine CCL2
Infectivity
Leishmania
Virulence
Macrophages
Kinetoplastida
biology.organism_classification
Virology
Chemokine CXCL10
Mice, Inbred C57BL
Infectious Diseases
Insect Science
biology.protein
Parasitology
Female
Chemokines
medicine.drug
Leishmania amazonensis
Subjects
Details
- ISSN :
- 18736254
- Volume :
- 115
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Acta tropica
- Accession number :
- edsair.doi.dedup.....d11900b5022aec116824d2c720e1f280