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Novel targets of sulforaphane in primary cardiomyocytes identified by proteomic analysis
- Source :
- PLoS ONE, Vol 8, Iss 12, p e83283 (2013), PLoS ONE
- Publication Year :
- 2013
- Publisher :
- Public Library of Science (PLoS), 2013.
-
Abstract
- Cardiovascular diseases represent the main cause of mortality in the industrialized world and the identification of effective preventive strategies is of fundamental importance. Sulforaphane, an isothiocyanate from cruciferous vegetables, has been shown to up-regulate phase II enzymes in cardiomyocytes and counteract oxidative stress-induced apoptosis. Aim of the present study was the identification and characterization of novel sulforaphane targets in cardiomyocytes applying a proteomic approach. Two-dimensional gel electrophoresis and mass spectrometry were used to generate protein profiles of primary neonatal rat cardiomyocytes treated and untreated with 5 µM sulforaphane for 1-48 h. According to image analysis, 64 protein spots were found as differentially expressed and their functional correlations were investigated using the MetaCore program. We mainly focused on 3 proteins: macrophage migration inhibitory factor (MIF), CLP36 or Elfin, and glyoxalase 1, due to their possible involvement in cardioprotection. Validation of the time-dependent differential expression of these proteins was performed by western blotting. In particular, to gain insight into the cardioprotective role of the modulation of glyoxalase 1 by sulforaphane, further experiments were performed using methylglyoxal to mimic glycative stress. Sulforaphane was able to counteract methylglyoxal-induced apoptosis, ROS production, and glycative stress, likely through glyoxalase 1 up-regulation. In this study, we reported for the first time new molecular targets of sulforaphane, such as MIF, CLP36 and glyoxalase 1. In particular, we gave new insights into the anti-glycative role of sulforaphane in cardiomyocytes, confirming its pleiotropic behavior in counteracting cardiovascular diseases.
- Subjects :
- Proteomics
Cardiotonic Agents
glyoxalase
Muscle Proteins
lcsh:Medicine
Apoptosis
Biology
primary cardiomyocyte
chemistry.chemical_compound
Isothiocyanates
Animals
Anticarcinogenic Agents
Myocytes, Cardiac
Rats, Wistar
lcsh:Science
Cells, Cultured
sulforaphane
Cardiomyocytes
ROS
proteomic
chemistry.chemical_classification
Reactive oxygen species
Multidisciplinary
Dose-Response Relationship, Drug
Methylglyoxal
lcsh:R
SULFORAPHANE
Pyruvaldehyde
Rats
Blot
Biochemistry
chemistry
Sulfoxides
Isothiocyanate
Macrophage migration inhibitory factor
REACTIVE OXYGEN SPECIES (ROS)
lcsh:Q
PROTEOMIC ANALYSIS
Reactive Oxygen Species
Sulforaphane
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....d15887a6c917c498fc8cfa61f5f62904