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Identification of a new reciprocal translocation in an AI bull by synaptonemal complex analysis, followed by chromosome painting
- Source :
- Cytogenetic and Genome Research. 121:245-248
- Publication Year :
- 2008
- Publisher :
- S. Karger AG, 2008.
-
Abstract
- An AI Ayrshire bull was subjected to cytogenetic examination due to lowered fertility. Preliminary Giemsa staining revealed a normal chromosome complement (60,XY) and G-banding did not allow us to draw a clear conclusion concerning an occurrence of chromosome rearrangement. Testicles were collected at slaughter and synaptonemal complex (SC) analysis revealed a large cross-shaped tetravalent configuration in pachytene spreads. No association between the tetravalent and XY bivalent was observed. Chromosome painting, with the use of bovine whole chromosome painting probes, conjugated with DAPI staining, facilitated a detailed description of the translocation rcp(2;4)(q45;q34). This study shows that post mortem analysis of synaptonemal complexes is a simple and useful tool for the preliminary detection of reciprocal translocation carriers.
- Subjects :
- Male
Genetics
Synaptonemal Complex
Chromosome
Chromosomal translocation
Chromosomal rearrangement
Biology
Translocation, Genetic
Giemsa stain
Bivalent (genetics)
Chromosome Painting
Staining
Microscopy, Electron
Synaptonemal complex
Animals
Cattle
Chromosome painting
Molecular Biology
Genetics (clinical)
Subjects
Details
- ISSN :
- 1424859X and 14248581
- Volume :
- 121
- Database :
- OpenAIRE
- Journal :
- Cytogenetic and Genome Research
- Accession number :
- edsair.doi.dedup.....d1598ed937186a0058a684f0c3974b63