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Myofibrillogenesis in live neonatal cardiomyocytes observed with hybrid two-photon excitation fluorescence-second harmonic generation microscopy
- Source :
- Journal of biomedical optics. 16(12)
- Publication Year :
- 2011
-
Abstract
- We developed a hybrid two-photon excitation fluorescence-second harmonic generation (TPEF-SHG) imaging system with an on-stage incubator for long-term live-cell imaging. Using the imaging system, we observed the addition of new sarcomeres during myofibrillogenesis while a cardiomyocyte was spreading on the substrate. The results suggest that the TPEF-SHG imaging system with an on-stage incubator is an effective tool for investigation of dynamic myofibrillogenesis.
- Subjects :
- Sarcomeres
Microscope
Materials science
animal structures
Research Papers: Imaging
Biomedical Engineering
Nanotechnology
Muscle Development
Sarcomere
law.invention
Biomaterials
Two-photon excitation microscopy
law
Microscopy
High harmonic generation
Animals
Myocytes, Cardiac
Cells, Cultured
Second-harmonic generation
Equipment Design
Second Harmonic Generation Microscopy
Fluorescence
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Rats
Microscopy, Fluorescence, Multiphoton
Animals, Newborn
Biophysics
Subjects
Details
- ISSN :
- 15602281
- Volume :
- 16
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of biomedical optics
- Accession number :
- edsair.doi.dedup.....d167535cf3821f09e4345d393fabd7e6