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Beyond the A‐layer: adsorption of lipopolysaccharides and characterization of bacteriophage‐insensitive mutants ofAeromonas salmonicidasubsp.salmonicida
- Source :
- Molecular Microbiology, Molecular Microbiology, Wiley, 2019, 112 (2), pp.667-677. ⟨10.1111/mmi.14308⟩
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- International audience; Aeromonas salmonicida subsp. salmonicida is a fish pathogen that causes furunculosis. Antibiotherapy used to treat furunculosis in fish has led to resistance. Virulent phages are increasingly seen as alternatives or complementary treatments against furunculosis in aquaculture environments. For phage therapy to be successful, it is essential to study the natural mechanisms of phage resistance in A. salmonicida subsp. salmonicida. Here, we generated bacteriophage-insensitive mutants (BIMs) of A. salmonicida subsp. salmonicida, using a myophage with broad host range and characterized them. Phage plaques were different depending on whether the A-layer surface array protein was expressed or not. The genome analysis of the BIMs helped to identify mutations in genes involved in the biogenesis of lipopolysaccharides (LPS) and on an uncharacterized gene (ASA_1998). The characterization of the LPS profile and gene complementation assays identified LPS as a phage receptor and confirmed the involvement of the uncharacterized protein ASA_1998 in phage infection. In addition, we confirmed that the presence of an A-layer at the bacterial surface could act as protection against phages. This study brings new elements into our understanding of the phage adsorption to A. salmonicida subsp. salmonicida cells.
- Subjects :
- Lipopolysaccharides
Phage therapy
viruses
animal diseases
medicine.medical_treatment
Mutant
Virus Attachment
Virulence
Aeromonas salmonicida
Biology
Microbiology
Bacteriophage
Fish Diseases
03 medical and health sciences
Bacterial Proteins
medicine
Animals
Bacteriophages
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Molecular Biology
Pathogen
030304 developmental biology
0303 health sciences
030306 microbiology
Fishes
Furunculosis
biology.organism_classification
Complementation
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Aeromonas
Mutation
Adsorption
Subjects
Details
- ISSN :
- 13652958 and 0950382X
- Volume :
- 112
- Database :
- OpenAIRE
- Journal :
- Molecular Microbiology
- Accession number :
- edsair.doi.dedup.....d1d2959a8293145e794c38777b515adc