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Loss of Detection of sgN Precedes Viral Abridged Replication in COVID-19-Affected Patients—A Target for SARS-CoV-2 Propagation
- Source :
- International Journal of Molecular Sciences; Volume 23; Issue 4; Pages: 1941
- Publication Year :
- 2022
-
Abstract
- The development of prophylactic agents against the SARS-CoV-2 virus is a public health priority in the search for new surrogate markers of active virus replication. Early detection markers are needed to follow disease progression and foresee patient negativization. Subgenomic RNA transcripts (with a focus on sgN) were evaluated in oro/nasopharyngeal swabs from COVID-19-affected patients with an analysis of 315 positive samples using qPCR technology. Cut-off Cq values for sgN (Cq < 33.15) and sgE (Cq < 34.06) showed correlations to high viral loads. The specific loss of sgN in home-isolated and hospitalized COVID-19-positive patients indicated negativization of patient condition, 3–7 days from the first swab, respectively. A new detection kit for sgN, gene E, gene ORF1ab, and gene RNAse P was developed recently. In addition, in vitro studies have shown that 2’-O-methyl antisense RNA (related to the sgN sequence) can impair SARS-CoV-2 N protein synthesis, viral replication, and syncytia formation in human cells (i.e., HEK-293T cells overexpressing ACE2) upon infection with VOC Alpha (B.1.1.7)-SARS-CoV-2 variant, defining the use that this procedure might have for future therapeutic actions against SARS-CoV-2.
- Subjects :
- SgE
Virus Replication
Giant Cells
Sensitivity and Specificity
Catalysis
Ribonuclease P
Viroporin Proteins
Inorganic Chemistry
QPCR methods
Limit of Detection
SgN
Nasopharynx
QPCR method
SARS-CoV-2 virus particle
Coronavirus Nucleocapsid Proteins
Humans
RNA, Antisense
Viral
Physical and Theoretical Chemistry
Antisense
Molecular Biology
Spectroscopy
SARS-CoV-2 virus particles
qPCR methods
2′-O-methyl antisense RNA
sgN
sgE
SARS-CoV-2
Organic Chemistry
COVID-19
General Medicine
Viral Load
Phosphoproteins
Computer Science Applications
HEK293 Cells
RNA, Viral
Social Isolation
RNA
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences; Volume 23; Issue 4; Pages: 1941
- Accession number :
- edsair.doi.dedup.....d1e93f40d38ee2f167ff12f57e9d47b2