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Loss of Detection of sgN Precedes Viral Abridged Replication in COVID-19-Affected Patients—A Target for SARS-CoV-2 Propagation

Authors :
Veronica Ferrucci
Pasqualino de Antonellis
Fabrizio Quarantelli
Fatemeh Asadzadeh
Francesca Bibbò
Roberto Siciliano
Carmen Sorice
Ida Pisano
Barbara Izzo
Carmela Di Domenico
Angelo Boccia
Maria Vargas
Biancamaria Pierri
Maurizio Viscardi
Sergio Brandi
Giovanna Fusco
Pellegrino Cerino
Livia De Pietro
Ciro Furfaro
Leonardo Antonio Napolitano
Giovanni Paolella
Lidia Festa
Stefania Marzinotto
Maria Concetta Conte
Ivan Gentile
Giuseppe Servillo
Francesco Curcio
Tiziana de Cristofaro
Francesco Broccolo
Ettore Capoluongo
Massimo Zollo
Ferrucci, V.
de Antonellis, P.
Quarantelli, F.
Asadzadeh, F.
Bibbo, F.
Siciliano, R.
Sorice, C.
Pisano, I.
Izzo, B.
Di Domenico, C.
Boccia, A.
Vargas, M.
Pierri, B.
Viscardi, M.
Brandi, S.
Fusco, G.
Cerino, P.
De Pietro, L.
Furfaro, C.
Napolitano, L. A.
Paolella, G.
Festa, L.
Marzinotto, S.
Conte, M. C.
Gentile, I.
Servillo, G.
Curcio, F.
de Cristofaro, T.
Broccolo, F.
Capoluongo, E.
Zollo, M.
Source :
International Journal of Molecular Sciences; Volume 23; Issue 4; Pages: 1941
Publication Year :
2022

Abstract

The development of prophylactic agents against the SARS-CoV-2 virus is a public health priority in the search for new surrogate markers of active virus replication. Early detection markers are needed to follow disease progression and foresee patient negativization. Subgenomic RNA transcripts (with a focus on sgN) were evaluated in oro/nasopharyngeal swabs from COVID-19-affected patients with an analysis of 315 positive samples using qPCR technology. Cut-off Cq values for sgN (Cq < 33.15) and sgE (Cq < 34.06) showed correlations to high viral loads. The specific loss of sgN in home-isolated and hospitalized COVID-19-positive patients indicated negativization of patient condition, 3–7 days from the first swab, respectively. A new detection kit for sgN, gene E, gene ORF1ab, and gene RNAse P was developed recently. In addition, in vitro studies have shown that 2’-O-methyl antisense RNA (related to the sgN sequence) can impair SARS-CoV-2 N protein synthesis, viral replication, and syncytia formation in human cells (i.e., HEK-293T cells overexpressing ACE2) upon infection with VOC Alpha (B.1.1.7)-SARS-CoV-2 variant, defining the use that this procedure might have for future therapeutic actions against SARS-CoV-2.

Details

Language :
English
Database :
OpenAIRE
Journal :
International Journal of Molecular Sciences; Volume 23; Issue 4; Pages: 1941
Accession number :
edsair.doi.dedup.....d1e93f40d38ee2f167ff12f57e9d47b2