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New Developments in Transcriptomic Analysis of Synovial Tissue
- Source :
- Frontiers in Medicine, Vol 7 (2020), Frontiers in Medicine
- Publication Year :
- 2020
- Publisher :
- Frontiers Media S.A., 2020.
-
Abstract
- Transcriptomic technologies are constantly changing and improving, resulting in an ever increasing understanding of gene expression in health and disease. These technologies have been used to investigate the pathological changes occurring in the joints of rheumatoid arthritis patients, leading to discoveries of disease mechanisms, and novel potential therapeutic targets. Microarrays were initially used on both whole tissue and cell subsets to investigate research questions, with bulk RNA sequencing allowing for further elaboration of these findings. A key example is the classification of pathotypes in rheumatoid arthritis using RNA sequencing that had previously been discovered using microarray and histology. Single-cell sequencing has now delivered a step change in understanding of the diversity and function of subpopulations of cells, in particular synovial fibroblasts. Future technologies, such as high resolution spatial transcriptomics, will enable step changes integrating single cell transcriptomic and geographic data to provide an integrated understanding of synovial pathology.
- Subjects :
- 0301 basic medicine
Microarray
Mini Review
Cell
synovium
Disease
Computational biology
Biology
fibroblast
Transcriptome
03 medical and health sciences
transcriptomics
0302 clinical medicine
stratification
Gene expression
medicine
skin and connective tissue diseases
030203 arthritis & rheumatology
lcsh:R5-920
pathotype
RNA
General Medicine
sequencing
single-cell
030104 developmental biology
medicine.anatomical_structure
Medicine
DNA microarray
lcsh:Medicine (General)
microarray
Function (biology)
Subjects
Details
- Language :
- English
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Frontiers in Medicine
- Accession number :
- edsair.doi.dedup.....d1eba467a7b178ef7a1cc514edf46f64
- Full Text :
- https://doi.org/10.3389/fmed.2020.00021/full