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Development of a Patient-Derived Xenograft (PDX) of Breast Cancer Bone Metastasis in a Zebrafish Model
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, Vol 17, Iss 8, p 1375 (2016), International Journal of Molecular Sciences, 17(8), 1375, International Journal of Molecular Sciences; Volume 17; Issue 8; Pages: 1375
- Publication Year :
- 2016
- Publisher :
- MDPI AG, 2016.
-
Abstract
- Bone metastasis is a complex process that needs to be better understood in order to help clinicians prevent and treat it. Xenografts using patient-derived material (PDX) rather than cancer cell lines are a novel approach that guarantees more clinically realistic results. A primary culture of bone metastasis derived from a 67-year-old patient with breast cancer was cultured and then injected into zebrafish (ZF) embryos to study its metastatic potential. In vivo behavior and results of gene expression analyses of the primary culture were compared with those of cancer cell lines with different metastatic potential (MCF7 and MDA-MB-231). The MCF7 cell line, which has the same hormonal receptor status as the bone metastasis primary culture, did not survive in the in vivo model. Conversely, MDA-MB-231 disseminated and colonized different parts of the ZF, including caudal hematopoietic tissues (CHT), revealing a migratory phenotype. Primary culture cells disseminated and in later stages extravasated from the vessels, engrafting into ZF tissues and reaching the CHT. Primary cell behavior reflected the clinical course of the patient’s medical history. Our results underline the potential for using PDX models in bone metastasis research and outline new methods for the clinical application of this in vivo model.
- Subjects :
- 0301 basic medicine
Pathology
medicine.medical_specialty
patient-derived xenograft
Cell
Bone Neoplasms
Breast Neoplasms
Article
Catalysis
lcsh:Chemistry
Inorganic Chemistry
bone metastasis
zebrafish model
breast cancer
03 medical and health sciences
0302 clinical medicine
Breast cancer
In vivo
medicine
Animals
Humans
Physical and Theoretical Chemistry
lcsh:QH301-705.5
Molecular Biology
Zebrafish
Spectroscopy
biology
business.industry
Organic Chemistry
Hematopoietic Tissue
Bone metastasis
General Medicine
medicine.disease
biology.organism_classification
Xenograft Model Antitumor Assays
Phenotype
Computer Science Applications
Disease Models, Animal
030104 developmental biology
medicine.anatomical_structure
lcsh:Biology (General)
lcsh:QD1-999
Cell culture
030220 oncology & carcinogenesis
Heterografts
Female
business
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....d2343d6cf9ae2e2bff75cf0acc42dce5
- Full Text :
- https://doi.org/10.3390/ijms17081375