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Development of a Patient-Derived Xenograft (PDX) of Breast Cancer Bone Metastasis in a Zebrafish Model

Authors :
Dino Amadori
Federica Recine
Laura Mercatali
Gabri van der Pluijm
Federico La Manna
Chiara Liverani
Andrea Giorgini
Arwin Groenewoud
Giacomo Miserocchi
Roberto Casadei
Toni Ibrahim
Alessandro De Vita
Roberto Biagini
Alberto Bongiovanni
Chiara Spadazzi
Ewa Snaar-Jagalska
Federica Pieri
Source :
International Journal of Molecular Sciences, International Journal of Molecular Sciences, Vol 17, Iss 8, p 1375 (2016), International Journal of Molecular Sciences, 17(8), 1375, International Journal of Molecular Sciences; Volume 17; Issue 8; Pages: 1375
Publication Year :
2016
Publisher :
MDPI AG, 2016.

Abstract

Bone metastasis is a complex process that needs to be better understood in order to help clinicians prevent and treat it. Xenografts using patient-derived material (PDX) rather than cancer cell lines are a novel approach that guarantees more clinically realistic results. A primary culture of bone metastasis derived from a 67-year-old patient with breast cancer was cultured and then injected into zebrafish (ZF) embryos to study its metastatic potential. In vivo behavior and results of gene expression analyses of the primary culture were compared with those of cancer cell lines with different metastatic potential (MCF7 and MDA-MB-231). The MCF7 cell line, which has the same hormonal receptor status as the bone metastasis primary culture, did not survive in the in vivo model. Conversely, MDA-MB-231 disseminated and colonized different parts of the ZF, including caudal hematopoietic tissues (CHT), revealing a migratory phenotype. Primary culture cells disseminated and in later stages extravasated from the vessels, engrafting into ZF tissues and reaching the CHT. Primary cell behavior reflected the clinical course of the patient’s medical history. Our results underline the potential for using PDX models in bone metastasis research and outline new methods for the clinical application of this in vivo model.

Details

ISSN :
14220067
Volume :
17
Database :
OpenAIRE
Journal :
International Journal of Molecular Sciences
Accession number :
edsair.doi.dedup.....d2343d6cf9ae2e2bff75cf0acc42dce5
Full Text :
https://doi.org/10.3390/ijms17081375