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Blueberry, combined with probiotics, alleviates non-alcoholic fatty liver diseaseviaIL-22-mediated JAK1/STAT3/BAX signaling
- Source :
- Food & Function. 9:6298-6306
- Publication Year :
- 2018
- Publisher :
- Royal Society of Chemistry (RSC), 2018.
-
Abstract
- Non-alcoholic fatty liver disease (NAFLD) is one of the most prevalent diseases worldwide. Blueberry, combined with probiotics (BP), might be a potential candidate for NAFLD treatment, due to its anti-inflammatory and anti-apoptotic properties. Here, we investigated whether the anti-inflammatory cytokine, IL-22, was involved in the therapeutic process of BP, using cell and rat models of NAFLD. Results indicated that BP significantly reduced lipid droplets and triglyceride (TG) accumulation in NAFLD cells. However, when IL-22 was deficient, the lipid droplets and TG content were significantly increased. In vivo, the serum parameters and pathological degree of NAFLD rats were significantly improved by BP, while IL-22 silencing significantly abolished the BP effect. Immunohistochemistry, immunofluorescence, qRT-PCR, and western blotting showed that the NAFLD group expressed significantly lower levels of IL-22, JAK1, and STAT3, and higher levels of BAX, than the normal group. Furthermore, BP significantly elevated the levels of IL-22, JAK1 and STAT3, and reduced the level of BAX in NAFLD, while IL-22 silencing prevented BP from restoring the expression of JAK1, STAT3, and BAX. We conclude that IL-22 is vital for the therapeutic effect of BP, and acts via activation of JAK1/STAT3 signaling and inhibition of the apoptosis factor BAX, which makes IL-22 a promising target for therapy of NAFLD.
- Subjects :
- Male
STAT3 Transcription Factor
0301 basic medicine
medicine.medical_specialty
medicine.medical_treatment
Blueberry Plants
Cell
03 medical and health sciences
chemistry.chemical_compound
Non-alcoholic Fatty Liver Disease
Internal medicine
Lipid droplet
medicine
Animals
Humans
Gene silencing
Rats, Wistar
STAT3
bcl-2-Associated X Protein
biology
Triglyceride
business.industry
Interleukins
Probiotics
Fatty liver
Janus Kinase 1
General Medicine
medicine.disease
digestive system diseases
Rats
Fruit and Vegetable Juices
030104 developmental biology
Endocrinology
medicine.anatomical_structure
Cytokine
chemistry
Apoptosis
biology.protein
business
Signal Transduction
Food Science
Subjects
Details
- ISSN :
- 2042650X and 20426496
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Food & Function
- Accession number :
- edsair.doi.dedup.....d26abc9c88a58a7266f4640b18099e90