Back to Search
Start Over
Broad anti–SARS-CoV-2 antibody immunity induced by heterologous ChAdOx1/mRNA-1273 vaccination
- Publication Year :
- 2022
- Publisher :
- Umeå universitet, Institutionen för klinisk mikrobiologi, 2022.
-
Abstract
- Heterologous prime-boost immunization strategies have the potential to augment COVID-19 vaccine efficacy. We longitudinally profiled severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S)–specific serological and memory B cell (MBC) responses in individuals who received either homologous (ChAdOx1:ChAdOx1) or heterologous (ChAdOx1:mRNA-1273) prime-boost vaccination. Heterologous messenger RNA (mRNA) booster immunization induced higher serum neutralizing antibody and MBC responses against SARS-CoV-2 variants of concern (VOCs) compared with that of homologous ChAdOx1 boosting. Specificity mapping of circulating B cells revealed that mRNA-1273 boost immunofocused ChAdOx1-primed responses onto epitopes expressed on prefusion-stabilized S. Monoclonal antibodies isolated from mRNA-1273–boosted participants displayed overall higher binding affinities and increased breadth of reactivity against VOCs relative to those isolated from ChAdOx1-boosted individuals. Overall, the results provide molecular insight into the enhanced quality of the B cell response induced after heterologous mRNA booster vaccination.
- Subjects :
- Adult
Male
Infectious Medicine
COVID-19 Vaccines
Protein Conformation
Immunization, Secondary
Infektionsmedicin
Antibodies, Viral
complex mixtures
Immunogenicity, Vaccine
Memory B Cells
Protein Domains
Antibody Specificity
ChAdOx1 nCoV-19
Humans
Immunization Schedule
Multidisciplinary
SARS-CoV-2
Antibodies, Monoclonal
Immunology in the medical area
Middle Aged
Antibodies, Neutralizing
Immunologi inom det medicinska området
Spike Glycoprotein, Coronavirus
Female
2019-nCoV Vaccine mRNA-1273
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....d296884a441f60b313cc7b24ce89e622