Suppl. Figure 6 from Inhibition of REDD1 Sensitizes Bladder Urothelial Carcinoma to Paclitaxel by Inhibiting Autophagy

Restoration of REDD1 expression counteracts miR-22-mediated Taxol sensitivity. EJ and BIU87 cells were treated with Taxol and combinations of miR-22 mimics, miR-22 antagonist, shRNA-REDD1, and REDD1 overexpression vector. (A) Protein expression of REDD1, EEF2K, c-caspase 3, c-PARP, and LC3-I/II was determined by western blot. (B) CCK-8 was used to determine treatment effect on cell proliferation. (C) Flow cytometry was used to detect cell apoptosis. (D) Autophagy was assessed by immunofluorescence of LCB3 and quantification by flow cytometry. ***P