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Impact of Antibiotic Gut Exposure on the Temporal Changes in Microbiome Diversity
- Source :
- Antimicrobial Agents and Chemotherapy. 63
- Publication Year :
- 2019
- Publisher :
- American Society for Microbiology, 2019.
-
Abstract
- Although the global deleterious impact of antibiotics on the intestinal microbiota is well known, temporal changes in microbial diversity during and after an antibiotic treatment are still poorly characterized. We used plasma and fecal samples collected frequently during treatment and up to one month after from 22 healthy volunteers assigned to a 5-day treatment by moxifloxacin (n = 14) or no intervention (n = 8). Moxifloxacin concentrations were measured in both plasma and feces, and bacterial diversity was determined in feces by 16S rRNA gene profiling and quantified using the Shannon index and number of operational taxonomic units (OTUs). Nonlinear mixed effect models were used to relate drug pharmacokinetics and bacterial diversity over time. Moxifloxacin reduced bacterial diversity in a concentration-dependent manner, with a median maximal loss of 27.5% of the Shannon index (minimum [min], 17.5; maximum [max], 27.7) and 47.4% of the number of OTUs (min, 30.4; max, 48.3). As a consequence of both the long fecal half-life of moxifloxacin and the susceptibility of the gut microbiota to moxifloxacin, bacterial diversity indices did not return to their pretreatment levels until days 16 and 21, respectively. Finally, the model characterized the effect of moxifloxacin on bacterial diversity biomarkers and provides a novel framework for analyzing antibiotic effects on the intestinal microbiome.
- Subjects :
- Adult
Male
medicine.drug_class
Moxifloxacin
Antibiotics
Biology
Gut flora
Microbiology
Feces
Young Adult
03 medical and health sciences
Diversity index
RNA, Ribosomal, 16S
medicine
Humans
Pharmacology (medical)
Microbiome
030304 developmental biology
Pharmacology
0303 health sciences
Bacteria
030306 microbiology
Microbiota
Sequence Analysis, DNA
Middle Aged
biology.organism_classification
medicine.disease
Healthy Volunteers
Anti-Bacterial Agents
Gastrointestinal Microbiome
3. Good health
Infectious Diseases
Metagenomics
Female
human activities
Dysbiosis
medicine.drug
Subjects
Details
- ISSN :
- 10986596 and 00664804
- Volume :
- 63
- Database :
- OpenAIRE
- Journal :
- Antimicrobial Agents and Chemotherapy
- Accession number :
- edsair.doi.dedup.....d2e7205edeb1574b74ab591b7ec567c5