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Ciliary neuropeptidergic signaling dynamically regulates excitatory synapses in postnatal neocortical pyramidal neurons
- Source :
- eLife, eLife, Vol 10 (2021)
- Publication Year :
- 2021
- Publisher :
- eLife Sciences Publications, Ltd, 2021.
-
Abstract
- Primary cilia are compartmentalized sensory organelles present on the majority of neurons in the mammalian brain throughout adulthood. Recent evidence suggests that cilia regulate multiple aspects of neuronal development, including the maintenance of neuronal connectivity. However, whether ciliary signals can dynamically modulate postnatal circuit excitability is unknown. Here we show that acute cell-autonomous knockdown of ciliary signaling rapidly strengthens glutamatergic inputs onto cultured rat neocortical pyramidal neurons and increases spontaneous firing. This increased excitability occurs without changes to passive neuronal properties or intrinsic excitability. Further, the neuropeptide receptor somatostatin receptor 3 (SSTR3) is localized nearly exclusively to excitatory neuron cilia both in vivo and in culture, and pharmacological manipulation of SSTR3 signaling bidirectionally modulates excitatory synaptic inputs onto these neurons. Our results indicate that ciliary neuropeptidergic signaling dynamically modulates excitatory synapses and suggest that defects in this regulation may underlie a subset of behavioral and cognitive disorders associated with ciliopathies.
- Subjects :
- Male
Patch-Clamp Techniques
QH301-705.5
Science
Neuropeptide
Sensory system
Neocortex
SSTR3
Biology
Ciliopathies
General Biochemistry, Genetics and Molecular Biology
Synapse
Glutamatergic
synapse
Organelle
Somatostatin receptor 3
Animals
Rats, Long-Evans
Receptors, Somatostatin
Biology (General)
neuropeptide
Cells, Cultured
Gene knockdown
General Immunology and Microbiology
General Neuroscience
Cilium
Pyramidal Cells
cilia
General Medicine
Cell Biology
Cortex (botany)
cortex
Synapses
Excitatory postsynaptic potential
Medicine
Rat
Female
Neuroscience
Research Article
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 2050084X
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- eLife
- Accession number :
- edsair.doi.dedup.....d2ede2146c014c1fe36dd494c99251d9