Quantitative deviating effects of maple syrup extract supplementation on the hepatic gene expression of mice fed a high-fat diet

cope Maple syrup contains various polyphenols and we investigated the effects of a polyphenol-rich maple syrup extract (MSXH) on the physiology of mice fed a high-fat diet (HFD). Methods and results The mice fed a low-fat diet (LFD), an HFD, or an HFD supplemented with 0.02% (002MSXH) or 0.05% MSXH (005MSXH) for 4 weeks. Global gene expression analysis of the liver was performed, and the differentially expressed genes were classified into three expression patterns; pattern A (LFD 002MSXH = 005MSXH, LFD > HFD 005MSXH, LFD > HFD = 002MSXH 002MSXH HFD 005MSXH). Pattern A was enriched in glycolysis, fatty acid metabolism, and folate metabolism. Pattern B was enriched in tricarboxylic acid cycle while pattern C was enriched in gluconeogenesis, cholesterol metabolism, amino acid metabolism, and endoplasmic reticulum stress-related event. Conclusion Our study suggested that the effects of MSXH ingestion showed (1) dose-dependent pattern involved in energy metabolisms and (2) reversely pattern involved in stress responses. This article is protected by copyright. All rights reserved