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Pulmonary pathobiology induced by zinc oxide nanoparticles in mice: A 24-hour and 28-day follow-up study
- Source :
- Toxicology and Applied Pharmacology. 327:13-22
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Inhaled zinc oxide nanoparticles (ZnONPs) have high deposition rates in the alveolar region of the lungs; however, the adverse health effects of ZnONPs on the respiratory system are unclear. Herein, pathobiological responses of the respiratory system of mice that received intratracheal administration of ZnONPs were investigated by a combination of molecular and imaging (SPECT and CT) approaches. Also, normal BEAS-2B and adenocarcinoma A549 cells were used to confirm the results in mice. First, female BALB/c mice were administrated a series of doses of 20-nm ZnONPs and were compared to the phosphate-buffered saline control for 24-h and 28-day follow-up observations. Field emission-scanning electron microscopy and an energy-dispersive X-ray microanalysis were first used to characterize ZnONPs. After 24h, instilled ZnONPs had caused significant increases in lactic dehydrogenase (LDH) in bronchoalveolar lavage fluid (BALF) and 8-hydroxy-2'-deoxyguanosine (8-OHdG), caspase-3, and the p63 tumor marker in lung tissues (p
- Subjects :
- Lung Diseases
Pathology
medicine.medical_specialty
Pulmonary toxicity
Metal Nanoparticles
Inflammation
010501 environmental sciences
Toxicology
medicine.disease_cause
01 natural sciences
Mice
03 medical and health sciences
0302 clinical medicine
In vivo
medicine
Animals
Respiratory system
Lung
0105 earth and related environmental sciences
Tomography, Emission-Computed, Single-Photon
Pharmacology
A549 cell
Mice, Inbred BALB C
L-Lactate Dehydrogenase
medicine.diagnostic_test
Caspase 3
business.industry
Deoxyguanosine
respiratory system
Phosphoproteins
DNA-Binding Proteins
Bronchoalveolar lavage
medicine.anatomical_structure
8-Hydroxy-2'-Deoxyguanosine
A549 Cells
030220 oncology & carcinogenesis
Trans-Activators
Female
Zinc Oxide
medicine.symptom
business
Bronchoalveolar Lavage Fluid
Oxidative stress
Transcription Factors
Subjects
Details
- ISSN :
- 0041008X
- Volume :
- 327
- Database :
- OpenAIRE
- Journal :
- Toxicology and Applied Pharmacology
- Accession number :
- edsair.doi.dedup.....d344a4d2fd5bfd9f594eab9c48d47974