Back to Search
Start Over
Patterns of brachyury expression in chordomas
- Source :
- Annals of Diagnostic Pathology. 53:151760
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Introduction Chordomas are rare malignant midline tumors, presumed to arise from notochordal remnants. This was further suggested by the discovery of the brachyury in chordomas pathogenesis. Its immunohistochemical expression has become the principal adjunct in the diagnosis of chordomas. However, studies about brachyury expression in chordomas are not fully comparable, mainly because they use different primary antibodies. Thus, the aim of this study is to investigate the expression of brachyury expression in a series of chordomas in conjunction to clinicopathological characteristics and to review the relevant literature providing all the details needed in the immunohistochemical study of brachyury. Materials and methods This is a retrospective study of 62 chordomas, diagnosed over a 22-year period. No dedifferentiated or poorly differentiated cases were included. A monoclonal primary antibody (clone A-4) was used and brachyury expression was evaluated by the H-score. Clinicopathological parameters studied were age, sex, tumor localization, decalcification status and tissue age. Fetal notochords were used for comparison. Results Mean H-score of nuclear brachyury expression was 129.8. The tissue age significantly influenced brachyury expression, the older samples expressing less brachyury. Decalcification demonstrated a trend to weaken brachyury expression. Clinical characteristics were not correlated with the patterns of brachyury expression. Notochords were negative. Literature review reveals several polyclonal antibodies used and a positivity of 75%–100% in chordomas with even more variable results in notochords. Conclusion In chordomas, as in other tumor types, an uniformization of studies about brachyury expression is needed, by considering the clone used, and the decalcification and the age of the sample, given the growing importance of brachyury in diagnosis and therapeutic steps.
- Subjects :
- Adult
Fetal Proteins
Male
musculoskeletal diseases
0301 basic medicine
Brachyury
Pathology
medicine.medical_specialty
Notochord
Clone (cell biology)
Biology
Pathology and Forensic Medicine
Pathogenesis
03 medical and health sciences
0302 clinical medicine
Biomarkers, Tumor
Chordoma
medicine
Humans
Aged
Retrospective Studies
Poorly differentiated
Decalcification Technique
General Medicine
Middle Aged
Neoplasms, Germ Cell and Embryonal
medicine.disease
Immunohistochemistry
Clone Cells
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Monoclonal
Female
T-Box Domain Proteins
Subjects
Details
- ISSN :
- 10929134
- Volume :
- 53
- Database :
- OpenAIRE
- Journal :
- Annals of Diagnostic Pathology
- Accession number :
- edsair.doi.dedup.....d348dc30c92b1f68b8d4b92e038c8f69