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RhoA regulates lipopolysaccharide‑induced lung cell injury via the Wnt/β‑catenin pathway
- Source :
- Molecular medicine reports. 16(6)
- Publication Year :
- 2016
-
Abstract
- Ras homolog family member A (RhoA) has been reported to be involved in numerous biological processes; however, the effects of RhoA on acute lung injury (ALI) have yet to be reported. The present study aimed to explore how RhoA affects cell viability, reactive oxygen species (ROS) activity and cell apoptosis in a cell model of lipopolysaccharide (LPS)‑induced ALI. An MTT assay, flow cytometry, reverse transcription‑quantitative polymerase chain reaction and western blotting were used to determine the effects of RhoA on cell viability, apoptosis and ROS activity. The results demonstrated that RhoA inactivation was able to promote cell viability, and decrease apoptosis and ROS activity of LPS‑treated cells. The results of western blotting indicated that RhoA activated the downstream Wnt/β‑catenin signaling pathway and inhibited the expression of apoptotic factors. These findings suggested that RhoA may be involved in ALI progression and could be a novel therapeutic target for this disease.
- Subjects :
- 0301 basic medicine
Lipopolysaccharides
Cancer Research
RHOA
Cell
Acute Lung Injury
Apoptosis
Lung injury
Biochemistry
03 medical and health sciences
0302 clinical medicine
Cell Line, Tumor
Genetics
medicine
Humans
Viability assay
RNA, Small Interfering
Molecular Biology
Wnt Signaling Pathway
biology
Wnt signaling pathway
Cell cycle
Cell biology
030104 developmental biology
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Catenin
Gene Knockdown Techniques
biology.protein
Cancer research
Molecular Medicine
Reactive Oxygen Species
rhoA GTP-Binding Protein
Subjects
Details
- ISSN :
- 17913004
- Volume :
- 16
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Molecular medicine reports
- Accession number :
- edsair.doi.dedup.....d3762e3271c001db16c73334b04541b3