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Critical analysis of current methods for assessing the in vitro antioxidant and antibacterial activity of plant extracts
- Source :
- Food Chemistry. 172:814-822
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Natural product research is an active branch of science, driven by the increased value placed on individual health and well-being. Many naturally-occurring phytochemicals in plants, fruits and vegetables have been reported to exhibit antioxidant and antibacterial activity; often touted as being beneficial for human health. In vitro screening is a common practice in many research laboratories as a means of rapidly assessing these properties. However, the methods used by many are not necessarily optimal; a result of poor standardization, redundant assays and/or outdated methodology. This review primarily aims to give a better understanding in the selection of in vitro assays, with emphasis placed on some common assays such as the total phenolic content assay, free radical scavenging activity, disc-diffusion and broth microdilution. This includes a discussion on the reasons for choosing a particular assay, its strengths and weaknesses, ways to improve the accuracy of results and alternative assays.
- Subjects :
- Free Radical Scavenging Activity
Antioxidant
Plant Extracts
Chemistry
business.industry
medicine.medical_treatment
Broth microdilution
In vitro toxicology
Free Radical Scavengers
General Medicine
In Vitro Techniques
Antioxidants
In vitro
Anti-Bacterial Agents
Analytical Chemistry
Biotechnology
Toxicology
Individual health
Phenols
medicine
Humans
Natural Product Research
Antibacterial activity
business
Food Science
Subjects
Details
- ISSN :
- 03088146
- Volume :
- 172
- Database :
- OpenAIRE
- Journal :
- Food Chemistry
- Accession number :
- edsair.doi.dedup.....d38b890ff635625768ef76651c05bf08
- Full Text :
- https://doi.org/10.1016/j.foodchem.2014.09.141