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An Enzyme Immune Assay for Serum Anti-Acetaldehyde Adduct Antibody Using Low-Density Lipoprotein Adduct and Its Significance in Alcoholic Liver Injury
- Source :
- Alcoholism: Clinical & Experimental Research. 22:150
- Publication Year :
- 1998
- Publisher :
- Wiley, 1998.
-
Abstract
- An acetaldehyde (AcH) adduct was prepared using rabbit low-density lipoprotein as carrier proteins. An antibody against this adduct was raised in Watanabe heritable hyperlipidemic rabbits and cross-reacted with human low-density lipoprotein and bovine serum albumin adducts. Using this antibody, serum anti-AcH-adduct antibody levels were measured by a direct ELISA method in 56 Japanese adults (healthy adults and patients with nonalcoholic gastrointestinal diseases, alcoholic liver injury, or alcoholic pancreatitis). The antibody level (mean +/- SD) was 22 +/- 10 microg/ml in healthy adults, 22 +/- 11 microg/ml in nonalcoholic gastrointestinal diseases, and 16 +/- 13 microg/ml in alcoholic pancreatitis. These antibody levels tended to increase with the progression of alcoholic liver injury, starting from fatty liver via hepatitis to cirrhosis, 29 +/- 24 microg/ml in fatty liver, 35 +/- 29 microg/ml in alcoholic hepatitis, and 46 +/- 54 microg/ml in alcoholic cirrhosis. The antibody level in patients taking 100 g or more of ethanol per day tended to be higher, compared with those in people taking less ethanol. A follow-up observation revealed that alcohol abstinence after hospitalization raised serum anti-AcH-adduct antibody level in some patients and kept it constantly low in other patients. The immunohistochemical study using the anti-AcH-adduct antibody revealed the presence of adduct-like substance in hepatocytes of liver biopsy specimens obtained from patients with alcoholic liver disease. The results indicate that the anti-AcH-adduct antibody may be associated with the progress of alcoholic liver diseases.
- Subjects :
- Adult
Male
medicine.medical_specialty
Alcoholic liver disease
Cirrhosis
Pancreatitis, Alcoholic
Alcoholic hepatitis
Medicine (miscellaneous)
Acetaldehyde
Toxicology
Antibodies
Immunoenzyme Techniques
chemistry.chemical_compound
Internal medicine
medicine
Animals
Humans
Liver Diseases, Alcoholic
Aged
Aged, 80 and over
Hepatitis
Liver injury
biology
business.industry
Fatty liver
Middle Aged
medicine.disease
Lipoproteins, LDL
Psychiatry and Mental health
Endocrinology
Liver
chemistry
Low-density lipoprotein
biology.protein
Cattle
Female
Rabbits
Antibody
business
Subjects
Details
- ISSN :
- 01456008
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Alcoholism: Clinical & Experimental Research
- Accession number :
- edsair.doi.dedup.....d3ab940dcdca0b4533ff0287901c1703