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Trimellitic anhydride induces low-grade mast cell degranulation without specific IgE
- Source :
- Toxicology Reports, Toxicology Reports, Vol 3, Iss, Pp 701-707 (2016)
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Objectives: Low-molecular-weight (LMW) substances are known to be causative agents of occupational asthma (OA) and occupational rhinitis (OR). Although most LMW substances are irritants or allergens, some can cause immediate type immunoglobulin E (IgE)-mediated allergic reactions. Trimellitic anhydride (TMA) is one such LMW substance, which is known as an immunological sensitizer. However, the exact molecular biological details of the effects of TMA remain unclear. Methods: We measured the β-hexosaminidase release from mast cells after directly exposing the cells to various LMW substances. The tyrosine phosphorylation of whole cellular molecules and the phosphorylation of extracellular signal-regulated kinase (ERK) were assessed by immunoblot assay. Results: Among the LMW substances tested, only TMA induced β-hexosaminidase release. However, the mast cell degranulation induced by TMA was lower than that induced by an antigen or a calcium ionophore. Moreover, the pattern of tyrosine phosphorylation of whole cellular molecules was quite different between IgE-mediated antigen stimulation and TMA exposure. The TMA effect on mast cells was independent of not only IgE but also Ca2+ influx. ERK phosphorylation was not detected in mast cells exposed to TMA. Conclusions: TMA induced mild degranulation of mast cells without IgE, even though the phosphorylation of ERK was not detected. This reaction suggests that TMA affects humans even upon first exposure. Therefore, it is imperative to avoid human exposure to high concentrations of TMA. In order to stop the development of severe asthma in individuals with OR, we need to be able to identify cases of OR caused by TMA as soon as possible. Keywords: Occupational rhinitis, Respiratory hypersensitivity, Trimellitic anhydride, Mast cell
- Subjects :
- 0301 basic medicine
Health, Toxicology and Mutagenesis
TMA, trimellitic anhydride
HMW, high molecular weight
Toxicology
Immunoglobulin E
Article
LMW, low molecular weight
Mast cell
OR, occupational rhinitis
03 medical and health sciences
Trimellitic anhydride
chemistry.chemical_compound
lcsh:RA1190-1270
immune system diseases
PD, piecemeal degranulation
hemic and lymphatic diseases
medicine
Occupational rhinitis
neoplasms
IgE, immunoglobulin E
lcsh:Toxicology. Poisons
IgE, immediate immunoglobulin E
OA, occupational asthma
biology
Degranulation
Respiratory Hypersensitivity
food and beverages
DNP, dinitrophenylated
medicine.disease
IL, interleukin
IgE - Immunoglobulin E
030104 developmental biology
medicine.anatomical_structure
chemistry
Respiratory hypersensitivity
Immunology
biology.protein
BSA, bovine serum albumin
Occupational asthma
Subjects
Details
- ISSN :
- 22147500
- Volume :
- 3
- Database :
- OpenAIRE
- Journal :
- Toxicology Reports
- Accession number :
- edsair.doi.dedup.....d3b9cc1749369f942ea51084f7403789
- Full Text :
- https://doi.org/10.1016/j.toxrep.2016.09.001