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Loss of Hippocampal CA3 Pyramidal Neurons in Mice Lacking STAM1
- Source :
- Molecular and Cellular Biology. 21:3807-3819
- Publication Year :
- 2001
- Publisher :
- Informa UK Limited, 2001.
-
Abstract
- STAM1, a member of the STAM (signal transducing adapter molecule) family, has a unique structure containing a Src homology 3 domain and ITAM (immunoreceptor tyrosine-based activation motif). STAM1 was previously shown to be associated with the Jak2 and Jak3 tyrosine kinases and to be involved in the regulation of intracellular signal transduction mediated by interleukin-2 (IL-2) and granulocyte-macrophage colony-stimulating factor (GM-CSF) in vitro. Here we generated mice lacking STAM1 by using homologous recombination with embryonic stem cells. STAM1/ mice were morphologically indistinguishable from their littermates at birth. However, growth retardation in the third week after birth was observed for the STAM1/ mice. Unexpectedly, despite the absence of STAM1, hematopoietic cells, including T- and B-lymphocyte and other hematopoietic cell populations, developed normally and responded well to several cytokines, including IL-2 and GM-CSF. However, histological analyses revealed the disappearance of hippocampal CA3 pyramidal neurons in STAM1/ mice. Furthermore, we observed that primary hippocampal neurons derived from STAM1/ mice are vulnerable to cell death induced by excitotoxic amino acids or an NO donor. These data suggest that STAM1 is dispensable for cytokine-mediated signaling in lymphocytes but may be involved in the survival of hippocampal CA3 pyramidal neurons.<br />抗酸化系遺伝子改変動物を用いた母体糖尿病による胎児異常発生抑止効果に関する研究
- Subjects :
- Male
Cell signaling
T-Lymphocytes
Gene Expression
Hippocampus
Mice
Seizures
Mammalian Genetic Models with Minimal or Complex Phenotypes
Animals
Receptor
Molecular Biology
Cells, Cultured
STAT5
Adaptor Proteins, Signal Transducing
Mice, Knockout
Neurons
Genetics
Kainic Acid
Janus kinase 2
Behavior, Animal
Endosomal Sorting Complexes Required for Transport
biology
Brain
Cell Biology
Phosphoproteins
Cell biology
Mice, Inbred C57BL
Fertility
Phenotype
Gene Targeting
biology.protein
Interleukin-2
Phosphorylation
Female
Disease Susceptibility
Signal transduction
Platelet-derived growth factor receptor
Subcellular Fractions
Proto-oncogene tyrosine-protein kinase Src
Subjects
Details
- ISSN :
- 10985549
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Molecular and Cellular Biology
- Accession number :
- edsair.doi.dedup.....d3c1c781c8ed55f3596efd2a330b856a