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Cuticular Structure Proteomics in the Pea Aphid Acyrthosiphon pisum Reveals New Plant Virus Receptor Candidates at the Tip of Maxillary Stylets
- Source :
- Journal of Proteome Research, Journal of Proteome Research, American Chemical Society, 2020, 19 (3), pp.1319-1337. ⟨10.1021/acs.jproteome.9b00851⟩, Journal of Proteome Research, American Chemical Society, 2020, ⟨10.1021/acs.jproteome.9b00851⟩
- Publication Year :
- 2020
- Publisher :
- HAL CCSD, 2020.
-
Abstract
- International audience; Aphids are phloem-feeding insects known as major pests in agriculture that are able to transmit hundreds of plant viruses. The majority of these viruses, classified as noncirculative, are retained and transported on the inner surface of the cuticle of the needle-like mouthparts while the aphids move from plant to plant. Identification of receptors of viruses within insect vectors is a key challenge because they are promising targets for alternative control strategies. The acrostyle, an organ discovered earlier within the common food/salivary canal at the tip of aphid maxillary stylets, displays proteins at the cuticle-fluid interface, some of which are receptors of noncirculative viruses. To assess the presence of stylet- and acrostyle-specific proteins and identify putative receptors, we have developed a comprehensive comparative analysis of the proteomes of four cuticular anatomical structures of the pea aphid, stylets, antennae, legs, and wings. In addition, we performed systematic immunolabeling detection of the cuticular proteins identified by mass spectrometry in dissected stylets. We thereby establish the first proteome of stylets of an insect and determine the minimal repertoire of the cuticular proteins composing the acrostyle. Most importantly, we propose a short list of plant virus receptor candidates, among which RR-1 proteins are remarkably predominant. The data are available via ProteomeXchange (PXD016517).
- Subjects :
- 0301 basic medicine
media_common.quotation_subject
[SDV]Life Sciences [q-bio]
Insect
Biology
stylets
Proteomics
Biochemistry
virus receptor
03 medical and health sciences
proteomics
Plant virus
ComputingMilieux_MISCELLANEOUS
media_common
Aphid
030102 biochemistry & molecular biology
Virus receptor
[SDV.BA]Life Sciences [q-bio]/Animal biology
fungi
food and beverages
General Chemistry
biology.organism_classification
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Arthropod mouthparts
Cell biology
Acyrthosiphon pisum
[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology
030104 developmental biology
aphid
cuticular proteins
Proteome
acrostyle
cuticle
[SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis
Subjects
Details
- Language :
- English
- ISSN :
- 15353893 and 15353907
- Database :
- OpenAIRE
- Journal :
- Journal of Proteome Research, Journal of Proteome Research, American Chemical Society, 2020, 19 (3), pp.1319-1337. ⟨10.1021/acs.jproteome.9b00851⟩, Journal of Proteome Research, American Chemical Society, 2020, ⟨10.1021/acs.jproteome.9b00851⟩
- Accession number :
- edsair.doi.dedup.....d3d1def4ed6e1e5ac12247d6e2c38971