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Genome-Wide Screen for Context-Dependent Tumor Suppressors Identified Using in Vivo Models for Neoplasia in Drosophila
- Source :
- G3: Genes|Genomes|Genetics, G3: Genes, Genomes, Genetics, Vol 10, Iss 9, Pp 2999-3008 (2020), Groth, C, Vaid, P, Khatpe, A, Prashali, N, Ahiya, A, Andrejeva, D, Chakladar, M, Nagarkar, S, Paul, R, Kelkar, D, Eichenlaub, T, Herranz, H, Sridhar, T S, Cohen, S M & Shashidhara, L S 2020, ' Genome-wide screen for context-dependent tumor suppressors identified using in vivo models for neoplasia in Drosophila ', G3: Genes, Genomes, Genetics, vol. 10, no. 9, pp. 2999-3008 . https://doi.org/10.1534/g3.120.401545
- Publication Year :
- 2020
- Publisher :
- Genetics Society of America, 2020.
-
Abstract
- Genetic approaches in Drosophila have successfully identified many genes involved in regulation of growth control as well as genetic interactions relevant to the initiation and progression of cancer in vivo. Here, we report on large-scale RNAi-based screens to identify potential tumor suppressor genes that interact with known cancer-drivers: the Epidermal Growth Factor Receptor and the Hippo pathway transcriptional cofactor Yorkie. These screens were designed to identify genes whose depletion drove tissue expressing EGFR or Yki from a state of benign overgrowth into neoplastic transformation in vivo. We also report on an independent screen aimed to identify genes whose depletion suppressed formation of neoplastic tumors in an existing EGFR-dependent neoplasia model. Many of the positives identified here are known to be functional in growth control pathways. We also find a number of novel connections to Yki and EGFR driven tissue growth, mostly unique to one of the two. Thus, resources provided here would be useful to all researchers who study negative regulators of growth during development and cancer in the context of activated EGFR and/or Yki and positive regulators of growth in the context of activated EGFR. Resources reported here are available freely for anyone to use.
- Subjects :
- EGFR
Hippo pathway
Context (language use)
Biology
QH426-470
medicine.disease_cause
law.invention
03 medical and health sciences
0302 clinical medicine
RNA interference
law
Neoplasms
medicine
Genetics
Animals
Drosophila Proteins
Neoplastic transformation
Genes, Tumor Suppressor
Epidermal growth factor receptor
Molecular Biology
Gene
Genetics (clinical)
030304 developmental biology
0303 health sciences
Hippo signaling pathway
Neoplasia
Mutant Screen Report
Nuclear Proteins
Cell biology
Drosophila melanogaster
030220 oncology & carcinogenesis
Tumorigenesis
biology.protein
Trans-Activators
Suppressor
Drosophila
Carcinogenesis
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 21601836
- Volume :
- 10
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- G3: Genes|Genomes|Genetics
- Accession number :
- edsair.doi.dedup.....d3ebf742441edda89f5f55d846b03d20