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Multiple roles of H2A.Z in regulating promoter chromatin architecture in human cells
- Source :
- Nature Communications, Vol 12, Iss 1, Pp 1-15 (2021), Nature Communications
- Publication Year :
- 2021
- Publisher :
- Nature Portfolio, 2021.
-
Abstract
- Chromatin accessibility of a promoter is fundamental in regulating transcriptional activity. The histone variant H2A.Z has been shown to contribute to this regulation, but its role has remained poorly understood. Here, we prepare high-depth maps of the position and accessibility of H2A.Z-containing nucleosomes for all human Pol II promoters in epithelial, mesenchymal and isogenic cancer cell lines. We find that, in contrast to the prevailing model, many different types of active and inactive promoter structures are observed that differ in their nucleosome organization and sensitivity to MNase digestion. Key aspects of an active chromatin structure include positioned H2A.Z MNase resistant nucleosomes upstream or downstream of the TSS, and a MNase sensitive nucleosome at the TSS. Furthermore, the loss of H2A.Z leads to a dramatic increase in the accessibility of transcription factor binding sites. Collectively, these results suggest that H2A.Z has multiple and distinct roles in regulating gene expression dependent upon its location in a promoter.<br />Histone variant H2A.Z has been suggested to contribute to the regulation of promoter accessibility. Here, the authors present high-depth maps of the position and accessibility of H2A.Z-containing nucleosomes for human Pol II promoters and provide evidence that H2A.Z has multiple and distinct roles in regulating gene expression dependent upon its location in a promoter.
- Subjects :
- Epigenomics
0301 basic medicine
Nucleosome organization
animal structures
Science
Gene Expression
General Physics and Astronomy
RNA polymerase II
Article
General Biochemistry, Genetics and Molecular Biology
Histones
03 medical and health sciences
Chromatin analysis
0302 clinical medicine
Cell Line, Tumor
Gene expression
Humans
Micrococcal Nuclease
Nucleosome
Promoter Regions, Genetic
Histone variants
Binding Sites
Multidisciplinary
biology
Chemistry
Promoter
General Chemistry
Chromatin
Nucleosomes
Cell biology
DNA binding site
030104 developmental biology
Histone
embryonic structures
biology.protein
RNA Polymerase II
Transcription
030217 neurology & neurosurgery
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 20411723
- Volume :
- 12
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....d409f621b8938c1aba8a6d3bb2088f25