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Copper and lactational hormones influence the CTR1 copper transporter in PMC42-LA mammary epithelial cell culture models
- Source :
- The Journal of Nutritional Biochemistry. 25:377-387
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- Adequate amounts of copper in milk are critical for normal neonatal development, however the mechanisms regulating copper supply to milk have not been clearly defined. PMC42-LA cell cultures representative of resting, lactating and suckled mammary epithelia were used to investigate the regulation of the copper uptake protein, CTR1. Both the degree of mammary epithelial differentiation (functionality) and extracellular copper concentration greatly impacted upon CTR1 expression and its plasma membrane association. In all three models (resting, lactating and suckling) there was an inverse correlation between extracellular copper concentration and the level of CTR1. Cell surface biotinylation studies demonstrated that as extracellular copper concentration increased membrane associated CTR1 was reduced. There was a significant increase in CTR1 expression (total and membrane associated) in the suckled gland model in comparison to the resting gland model, across all copper concentrations investigated (0-50 μM). Regulation of CTR1 expression was entirely post-translational, as quantitative real-time PCR analyses showed no change to CTR1 mRNA between all models and culture conditions. X-ray fluorescence microscopy on the differentiated PMC42-LA models revealed that organoid structures distinctively accumulated copper. Furthermore, as PMC42-LA cell cultures became progressively more specialised, successively more copper accumulated in organoids (resting
- Subjects :
- medicine.medical_specialty
Endocrinology, Diabetes and Metabolism
Clinical Biochemistry
chemistry.chemical_element
Biology
Biochemistry
Internal medicine
Lactation
medicine
Extracellular
Organoid
Humans
Metallothionein
Secretion
Mammary Glands, Human
Cation Transport Proteins
Molecular Biology
Cells, Cultured
Copper Transporter 1
Nutrition and Dietetics
Cell Membrane
fungi
Cell Differentiation
Epithelial Cells
Copper
Prolactin
Cell biology
Endocrinology
medicine.anatomical_structure
chemistry
Cell culture
Female
Hormone
Subjects
Details
- ISSN :
- 09552863
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- The Journal of Nutritional Biochemistry
- Accession number :
- edsair.doi.dedup.....d45043c85485fd166d9518f4e37e519c