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Na+/H+ exchange and pH regulation in the control of neutrophil chemokinesis and chemotaxis
- Source :
- American Journal of Physiology : Cell Physiology, 294, 2, pp. C526-34, American Journal of Physiology : Cell Physiology, 294, C526-34
- Publication Year :
- 2008
-
Abstract
- Large proton fluxes accompany cell migration, but their precise role remains unclear. We studied pH regulation during the course of chemokinesis and chemotaxis in human neutrophils stimulated by attractant peptides. Activation of cell motility by chemoattractants was accompanied by a marked increase in metabolic acid generation, attributable to energy consumption by the contractile machinery and to stimulation of the NADPH oxidase and the ancillary hexose monophosphate shunt. Despite the increase in acid production, the cytosol underwent a sizable alkalinization, caused by acceleration of Na+/H+exchange. The development of the alkalinization mirrored the increase in the rate of cell migration, suggesting a causal relationship. However, elimination of Na+/H+exchange by omission of external Na+or by addition of potent inhibitors was without effect on either chemokinesis or chemotaxis, provided the cytosolic pH remained near neutrality. At more acidic levels, cell motility was progressively inhibited. These observations suggest that Na+/H+exchange plays a permissive role in cell motility but is not required for the initiation or development of the migratory response. Chemokinesis also was found to be exquisitely sensitive to extracellular acidification. This property may account for the inability of neutrophils to access abscesses and solid tumors that have been reported to have inordinately low pH.
- Subjects :
- Sodium-Hydrogen Exchangers
Physiology
Neutrophils
Neutrophile
Motility
Chemokinesis
Biology
Cell Line
Metabolism, transport and motion [NCMLS 2]
Dogs
Proton transport
Animals
Humans
Microscopy, Interference
Enzyme Inhibitors
Cytoskeleton
Cation Transport Proteins
Cells, Cultured
Sodium-Hydrogen Exchanger 1
Chemotaxis
Sodium
NADPH Oxidases
Cell migration
Extracellular Fluid
Cell Biology
Hydrogen-Ion Concentration
Cell biology
Renal disorders [UMCN 5.4]
Biochemistry
Microscopy, Fluorescence
Ph regulation
Chemokines
Acids
Hydrogen
Subjects
Details
- ISSN :
- 03636143
- Volume :
- 294
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology : Cell Physiology
- Accession number :
- edsair.doi.dedup.....d452826b147d333101abe56a2f2f1e5f