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Biotin interferences

Authors :: Damien Gruson
Tatiana Roy
Benoît Kabamba-Mukadi
Julien Favresse
Anke Stoefs
Hector Rodriguez-Villalobos
Jean-Louis Bayart
Mélanie Closset
Catherine Fillée
Brussels Heritage Lab
Clinical Biology
UCL - SSS/IREC/MONT - Pôle Mont Godinne
UCL - SSS/IREC/MBLG - Pôle de Microbiologie médicale
UCL - SSS/IREC/SLUC - Pôle St.-Luc
UCL - SSS/IREC/EDIN - Pôle d'endocrinologie, diabète et nutrition
UCL - (MGD) Laboratoire de biologie clinique
UCL - (SLuc) Service de biochimie médicale
UCL - (SLuc) Service de microbiologie
Source :: Clinica chimica acta, Vol. 503, p. 107-112 (2020)
Publication Year :: 2020
Publisher :: Elsevier, 2020.
Abstract: BACKGROUND: Biotin has been reported to be a leading cause of interference on several immunoassay platforms using the streptavidin-biotin immobilization system. While biotin interferences have now been well characterized for several assays, only few data are available on their impact on serological markers of infectious viral diseases. METHODS: Overall, 10 healthy volunteers (HVs) received a single 100 mg dose of biotin to evaluate its effect on hepatitis B serological markers. Blood samples were taken several times before and after biotin intake. In addition, spiking experiments were applied to investigate biotin's impact on anti-HIV/p24 Ag and anti-HCV antibody levels. Several procedures designed to overcome this interference were evaluated. RESULTS: Biotin intake resulted in a false-negative anti-HBs immunological status (