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Single-cell kinase assays: opening a window onto cell behavior
- Source :
- Current opinion in biotechnology. 14(1)
- Publication Year :
- 2003
-
Abstract
- Recent advances in analytical techniques have made the performance of biochemical assays on individual mammalian cells possible. Of particular interest is the ability to measure the activation of kinases, enzymes with critical roles in virtually every aspect of cell physiology. Single-cell kinase assays promise to deliver a newfound understanding of the molecular mechanisms responsible for cellular control and behavior by revealing the dynamic nature of signal transduction networks in living cells. A recent exciting development is the potential to perform assays of multiple kinases simultaneously in a single cell.
- Subjects :
- MAPK/ERK pathway
Cell physiology
Electrophoresis
ABL
Kinase
Cells
Cell
Phosphotransferases
Biomedical Engineering
Bioengineering
Biology
Immunohistochemistry
Recombinant Proteins
Cell biology
Substrate Specificity
Enzyme Activation
medicine.anatomical_structure
Spectrometry, Fluorescence
Microscopy, Fluorescence
Ca2+/calmodulin-dependent protein kinase
medicine
Fluorescence Resonance Energy Transfer
Signal transduction
Protein kinase C
Biotechnology
Subjects
Details
- ISSN :
- 09581669
- Volume :
- 14
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Current opinion in biotechnology
- Accession number :
- edsair.doi.dedup.....d4761518a43d4cce75356e4c44373d79