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Transcutaneous vaccination using a hydrogel patch induces effective immune responses to tetanus and diphtheria toxoid in hairless rat
Transcutaneous vaccination using a hydrogel patch induces effective immune responses to tetanus and diphtheria toxoid in hairless rat
- Source :
- Journal of Controlled Release. 149:15-20
- Publication Year :
- 2011
- Publisher :
- Elsevier BV, 2011.
-
Abstract
- Transcutaneous immunization (TCI) targeting the Langerhans cells (LCs) of the epidermal layer is a promising needle-free, easy-to-use, and non-invasive vaccination method. We developed a hydrogel patch formulation to promote the penetration of antigenic proteins into the stratum corneum. Here, we investigated the characteristics of the immune responses induced by this vaccination method and the vaccine efficacy of TCI using a hydrogel patch containing tetanus and diphtheria toxoids. Our TCI system induced toxoid-specific IgG production in an antigen dose-, patch area-, and application period-dependent manner. Moreover, IgG subclass analysis indicated that our TCI predominantly elicited a Th2-type immune response rather than a Th1-type immune response. Importantly, our TCI system induced antigen-specific immune memory based on the booster effect and showed potent efficacy, comparable to that of subcutaneous immunization in toxin-challenge experiments. On the basis of these results, we are now performing translational research to apply TCI for tetanus and diphtheria.
- Subjects :
- Diphtheria Toxoid
Transdermal patch
Chemistry, Pharmaceutical
Guinea Pigs
Rats, Hairless
Transdermal Patch
Pharmaceutical Science
Enzyme-Linked Immunosorbent Assay
chemical and pharmacologic phenomena
complex mixtures
Mice
Th2 Cells
Immune system
Tetanus Toxin
Antigen
Tetanus Toxoid
medicine
Animals
Diphtheria Toxin
Diphtheria toxin
Mice, Inbred ICR
Tetanus
business.industry
Diphtheria
Vaccination
Toxoid
Hydrogels
Th1 Cells
medicine.disease
Rats
Immunoglobulin G
Immunology
Female
business
Immunologic Memory
Subjects
Details
- ISSN :
- 01683659
- Volume :
- 149
- Database :
- OpenAIRE
- Journal :
- Journal of Controlled Release
- Accession number :
- edsair.doi.dedup.....d48bf838ddbe0fd91b34d4c3d2a22caa
- Full Text :
- https://doi.org/10.1016/j.jconrel.2010.05.012