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Transcutaneous vaccination using a hydrogel patch induces effective immune responses to tetanus and diphtheria toxoid in hairless rat

Transcutaneous vaccination using a hydrogel patch induces effective immune responses to tetanus and diphtheria toxoid in hairless rat

Authors :
Yasuo Yoshioka
Naoki Okada
Yohei Mukai
Yumiko Ishii
Shinsaku Nakagawa
Fumio Kamiyama
Kazuhiko Matsuo
Ying-Shu Quan
Source :
Journal of Controlled Release. 149:15-20
Publication Year :
2011
Publisher :
Elsevier BV, 2011.

Abstract

Transcutaneous immunization (TCI) targeting the Langerhans cells (LCs) of the epidermal layer is a promising needle-free, easy-to-use, and non-invasive vaccination method. We developed a hydrogel patch formulation to promote the penetration of antigenic proteins into the stratum corneum. Here, we investigated the characteristics of the immune responses induced by this vaccination method and the vaccine efficacy of TCI using a hydrogel patch containing tetanus and diphtheria toxoids. Our TCI system induced toxoid-specific IgG production in an antigen dose-, patch area-, and application period-dependent manner. Moreover, IgG subclass analysis indicated that our TCI predominantly elicited a Th2-type immune response rather than a Th1-type immune response. Importantly, our TCI system induced antigen-specific immune memory based on the booster effect and showed potent efficacy, comparable to that of subcutaneous immunization in toxin-challenge experiments. On the basis of these results, we are now performing translational research to apply TCI for tetanus and diphtheria.

Details

ISSN :
01683659
Volume :
149
Database :
OpenAIRE
Journal :
Journal of Controlled Release
Accession number :
edsair.doi.dedup.....d48bf838ddbe0fd91b34d4c3d2a22caa
Full Text :
https://doi.org/10.1016/j.jconrel.2010.05.012