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Fed batch enzymatic saccharification of food waste improves the sugar concentration in the hydrolysates and eventually the ethanol fermentation by Saccharomyces cerevisiae H058
- Source :
- Brazilian Archives of Biology and Technology v.55 n.2 2012, Brazilian Archives of Biology and Technology, Instituto de Tecnologia do Paraná (Tecpar), instacron:TECPAR, Brazilian Archives of Biology and Technology, Vol 55, Iss 2, Pp 183-192 (2012), Brazilian Archives of Biology and Technology, Volume: 55, Issue: 2, Pages: 183-192, Published: APR 2012
- Publication Year :
- 2012
- Publisher :
- Instituto de Tecnologia do Paraná - Tecpar, 2012.
-
Abstract
- The enzymatic hydrolysis of food waste by commercially available enzymes and the subsequent ethanol fermentation of the hydrolysates by Saccharomyces cerecisiae H058 were studied in this work. The optimum batch enzymatic conditions were found to be saccharification pH of 4.5, temperature of 55!, glucoamylase concentration of 120 u/g, α-amylase concentration of 10 u/g, solid-liquid ratio of 1: 0.75 (w/w). Fed batch hydrolysis process was started with a solid-liquid ratio of 1: 1 (w/w), with solid food waste added at time lapse of 2 h to get a final solid-liquid ratio of 1: 0.5 (w/w). After 4 h of reaction, the reducing sugar concentration reached 194.43 g/L with a enzymatic digestibility of 93.12%. Further fermentation of the batch and fed batch enzymatic hydrolysates, which contained reducing sugar concentration of 131.41 and 194.43 g/L respectively, was performed using Saccharomyces cerevisiae H058, 62.93 and 90.72 g/L ethanol was obtained within 48 h.
- Subjects :
- chemistry.chemical_classification
Multidisciplinary
biology
lcsh:Biotechnology
Food waste
Ethanol fermentation
Hydrolysates
biology.organism_classification
Saccharomyces
Hydrolysate
Reducing sugar
Hydrolysis
chemistry
Enzymatic hydrolysis
Ethanol production
lcsh:TP248.13-248.65
Fermentation
Food science
Sugar
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Brazilian Archives of Biology and Technology v.55 n.2 2012, Brazilian Archives of Biology and Technology, Instituto de Tecnologia do Paraná (Tecpar), instacron:TECPAR, Brazilian Archives of Biology and Technology, Vol 55, Iss 2, Pp 183-192 (2012), Brazilian Archives of Biology and Technology, Volume: 55, Issue: 2, Pages: 183-192, Published: APR 2012
- Accession number :
- edsair.doi.dedup.....d4dc52037fae8ec851c1067f1323830a